disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous in his stool. The patient had no pain but mild discomfort during bowel movements. The doctor noted inflammation of the lining of the rectum. The
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The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The SIM test
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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Increasing Fiber in Rice Pilaf Arcelay‚ Marilyn March 30‚ 2007 FOS 4041L Dr. Dixon Introduction Obesity is a word that everyone is currently familiar with. The media and health professionals have been working tirelessly to make the general public aware of its prevalence and detriments to society. With the staggering statistics of 32.2% prevalence in adults and a range of 13.9% to 18.9% prevalence in children and adolescents‚ these outstanding numbers stand
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Reyes Mykee Domingo Aaron Santos Ralph Reyes LBYMATB V26B Report Title Of Activity: Yogurt Making Date Performed: October 4‚ 2012 I. Introduction Last October 4‚ the group performed an activity that involved making our own yogurt. The group prepared the materials and followed the procedures to make the said yogurt. In the activity paper that was given‚ it dictated that during the yogurt making process‚ the bacteria underwent fermentation. “Fermentation is any process where microorganisms
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Laboratory(TFMB1001) Report Experiment Tittle Gram stain Experiment Date 24October2014 Submission Date 31October2014 Students Name Tittle Gram stain. Objectives Aim of this experiment is to differentiate between the two major categories of bacteria ‚ gram positive and gram negative. Through this experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink
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Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests
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food industry such as the production of cheese‚ bacteria takes a significant role on the wide variety of cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation
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