Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
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-Unknown Mixtures Lab- Purpose: The aim of this experiment was to find out the two substances of the unknown mixture from salt‚ sugar‚ dirt‚ and baking soda by looking at its properties. Hypothesis: Find the unknown substance by testing it out by heating it‚ pouring water‚ vinegar‚ and observing it . Procedure: 1) Materials- * 100ml of water * Vinegar * Bunsen Burner * 2 beakers * 1 spatula 2) First‚ do steps (3-6)to find out the properties of salt‚ sugar
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staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during the activity in lesson proper(flashcards) * iodine-
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Lab Report 1 Introduction: Proper chemical formulas entitle many challenges such as the Law of Multiple proportions that states that there may be more than one plausible mole ratio for the elements in that compound. However if we determine the mass of each element in the compound we will be able to get the true chemical formula. In this experiment‚ we used the law of definite proportions to find the chemical formula for a hydrated compound containing copper‚ chlorine‚ and water molecules
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the Earth and humans are known(6). This lab specifically does not require the knowledge of what each organism does when introduced to another living organism‚ like a human. Figuring out the type of bacteria could help with further experiments. The idea of the unknown bacteria lab is to show
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MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed‚ there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative
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Preliminary Unknown Report Alexis Grettenberger 800503431 Katie Young Alexis Grettenberger Biol 311 Lab 11/21/14 Preliminary Unknown Report Purpose: The purpose of this experiment was to identify a specific unknown bacterium that was isolated from a multitude of bacteria in a sample of contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that
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_____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the colonies formed: Yeasts: Moist‚ creamy bacteria-like‚ opaque‚ or pasty colonies on media. They reproduce by budding. (when they start budding‚ they cause infections) Molds (filamentous fungi): Fluffy cottony‚ woolly or powdery colonies on medium. They reproduce by sporulation. 2. Describe or
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Lab 11 Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology
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** Introduction : A medical microbiology lab performs testing on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology
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