MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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The purpose of this lab is to isolate a bacterial population from the normal throat flora. A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab
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difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are by and
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this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Determining the Unknown Microorganism from a Mixed Culture Mylah Ferland‚ Rivier University MICRO 206‚ March 16th‚ 2016 Introduction: An unknown bacterium experiment‚ this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al.‚ 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by
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Sampling of Gram-Positive Bacteria and Antibiotic Resistance Resting We thought it would be interesting to find out the different bacteria that grow on things we use on a daily basis and the level of antibiotic resistance that those bacteria have. We chose to swab the toilet seat in the MATC women’s bathroom and the ATM machine in the commons area on campus. We were certain the ATM would be dirtier than the toilet seat since the bathrooms get cleaned every day. The first step we took in testing
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Unknown Bacteria Lab Report Introduction The purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown organism in question and how the bacteria function. Over a two week period‚ eight prepared types of test media were provided to identify
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purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain‚ used
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