Interactions BigIdea 4 investigation 13 ENZYME ACTIVITY* How do abiotic or biotic factors influence the rates of enzymatic reactions? ■■BACKGROUND Enzymes speed up chemical reactions by lowering activation energy (that is‚ the energy needed for a reaction to begin). In every chemical reaction‚ the starting materials (the substrate(s) in the case of enzymes) can take many different paths to forming products. For each path‚ there is an intermediate or transitional product between reactants and
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Starch can be hydrolyzed into simpler carbohydrates by acids‚ various enzymes‚ or a combination of the two. The resulting fragments are known as dextrins. The extent of conversion is typically quantified by dextrose equivalent (DE)‚ which is roughly the fraction of the glycosidic bonds in starch that have been broken. These starch sugars are by far the most common starch based food ingredient and are used as sweetener in many drinks and foods. They include: Maltodextrin‚ a lightly hydrolyzed (DE
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Conclusion This lab was helpful in showing us how enzyme catalysis really happens in biology. These series of experiments have proven what the optimal conditions and which are not conducive to the functioning of Peroxidase. Table 7.1 was the trial experiment and was performed to observe the overall activity of the enzyme Peroxidase. Each of the three tube mixtures were filled with different concentrations of Peroxidase. As seen on the chart the mixture of tube six and seven had the fastest
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Identification of the Marker Enzymes Present in Different Fractions of a Chicken Liver Cell Priscilla Mariel M. Cadiz Biology Department‚ De La Salle University‚ 2401 Taft Ave‚ Manila‚ Philippines *Email: cadizpriscillamariel@yahoo.com Cell Fractionation allows the organelles to be studied in more depth and detailed. It is an important technique in Cell Biology because it enables to obtain precise information about the different structure and functions of the organelles. Certain organelles
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Value of the data • This is the first draft genome of Trametes villosa‚ a tropical white-rot Basidiomycota from the semiarid region of Brazil‚ promising for its production of ligninolytic enzymes. • T. villosa isolate CCMB561 is a good producer of lignin peroxidase‚ manganese peroxidase‚ and laccase‚ enzymes considered key for lignin degradation‚ providing a major advantage for its use in bioenergy research. • The draft genome will accelerate functional genomics research‚ helping to understand the
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that this is the bonafide project report of work carried out by Mr. M. RAVI TEJA (08311A2338) and Ms. V. MAYURI (08311A2358) at Department of Microbiology‚ Osmania University. The work incorporated in this report entitled “CLONING OF HORSE RADISH PEROXIDASE GENE IN E.coli FOR EXTRACELLULAR EXPRESSION”. Internal guide Head of Department External Examiner |Contents |Page No.
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Nonylphenol and bisphenol A binding studies with antioxidant enzymes through homology modeling and molecular docking methods M. Jayakanthan1#‚ R. Jubendradass2‚ Shereen Cynthia D’Cruz2‚ P. P. Mathur1‚2‚3* 1 Centre for Bioinformatics‚ School of Life Sciences‚ Pondicherry University‚ Pondicherry- 605 014‚ India 2 Department of Biochemistry and Molecular Biology‚ School of Life Sciences‚ Pondicherry University‚ Pondicherry - 605 014‚ India 3 KIIT University‚ Bhubaneswar - 751024‚ India
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UNIVERSITY OF DAR ES SALAAM COLLEGE OF NATURAL AND APPLIED SCIENCE Dept: Molecular biology and biotechnology Name: Ernest Medard Reg#:2010-04-00120 Degree program: Bsc. MBB BL 390: research project TITLE: production of Laccase and Pectinase enzymes by Schizophyllum spp and its application in dye decolorization Project supervisor: Dr. R. Masalu Lab scientist: Mr. Chuwa INTRODUCTION: Due to rapid industrialization and urbanization‚ a lot of chemicals including dyes are manufactured and used
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gear: lab coat‚ safety goggles‚ and safety gloves. It was best to work in pairs and one partner needed to be a timekeeper while the other one would record the data. The timekeeper then would announce every 5 second interval‚ beginning from when the enzyme was added to the tube. On the other hand‚ the recorder would read and record the absorbance from the spectrometer at the 5 second intervals. This was done for all four experiments so the absorbance could be recorded every 5 seconds for 60 seconds
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they can also be damaging; plants and animals maintain complex systems of multiple types of antioxidants‚ such as glutathione‚ vitamin C‚ vitamin A‚ and vitamin E as well as enzymes such as catalase‚ superoxide dismutase and various peroxidases. Insufficient levels of antioxidants‚ or inhibition of the antioxidant enzymes‚ cause oxidative stress and may damage or kill cells. Oxidative stress is damage to cell structure and cell function by overly reactive oxygen-containing molecules and chronic
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