• A Guaiacol Dye-Coupled Reaction Reports That Catalytic Activity of Peroxidase Isolated from Fresh Turnip (Brassica Rapa) Increases as Temperature Rises.
    experiment will investigate the effects of temperature on the enzyme kinetics – that is, the rate of an enzyme’s catalysis – of peroxidase isolated from turnip. Plant peroxidases are involved in lignin formation, which is part of the cell wall (Cosio and Dunand 1985). Turnip roots contain peroxidases...
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  • Finding the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer
    . Guaiacol is a dye that turns brown when oxygen is supplied. In this experiment, the tissue from a turnip will be homogenized and filtered to get most of the enzyme peroxidase. The peroxidase will mix with the hydrogen peroxide and the enzyme activity will be measured using the spectrometer. I think...
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  • Affects of Ph on Peroxidase Enzyme
    The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy, speeding up the reaction. The activity of...
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  • Peroxidase
    Publication No. 11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals, including bacteria, to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily...
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  • Bio Paper
    different trials we used the spectrophotometer to measure the absorbance of the reactions between guaiacol, H2O2, and the enzyme peroxidase. We use these to see how they influence the reaction rate of turnip peroxidase. We found that the pH solutions affected the absorbance of our product, and how they...
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  • Horseradish Peroxidase
    ) into water and another harmless compound. In this experiment we use, turnips and horseradish roots which are rich in the peroxidase to study the activity of this enzyme. The activity of peroxidase with change in temperature was highest at 320 Celsius and lowest at 40C. The activity of peroxidase was...
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  • Peroxidase Lab Report
    Determining the Catalytic Properties of the Enzyme Peroxidase Extracted from a Turnip Under the Conditions of Temperature, pH, Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30, 2013 ABSTRACT The enzyme, peroxidase, extracted from a turnip was tested for its...
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  • Temperature's Effect Against the Irreversible Denaturation of Peroxidase
    temperature on the activity of enzyme-catalyzed reactions, we made fifteen tubes that contained reaction buffer, hydrogen peroxide, turnip extract, and the dye. These reagents were placed in large bottles and were labeled with a sharpie. We gathered fifteen small test tubes for testing and three large test...
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  • Bio Lab Essay K101
    The reactions and Results of Using Enzyme Turnip Root Peroxidase Lab results from: Andrew Compton, Mickey. Results published 9/29/2012. TA: In this series of laboratory experiments, my lab partner and I were to conduct an experiment about the oxidation rate of the enzyme peroxidase in the...
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  • AP Investigation Lab #13 Enzyme Activity
    guiacol product indicator, and a neutral buffer. The guiacol is a naturally organic compound and is actually a phosphate. The other six test tubes (#3, 5, 6, 7, 8, and 10) had the enzyme turnip peroxidase and different pH solutions for each of the six test tubes. The different pH levels were 3, 5, 6...
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  • Meep
    In this experiment we tested how different PH solutions react to different products. Through different trials we used the spectrophotometer to measure the absorbance of the reactions between guaiacol, H2O2, and the enzyme peroxidase. An enzyme is a catalyst that speeds up an enzyme, which lowers...
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  • Enzyme
    ENZYME REACTIONS–Peroxidase I – BACKGROUND Introduction: In your discussion section, you have decided, or will decide, upon a particular tissue of a particular plant to examine. You probably chose either to compare it to turnip root (for which we already have information; turnip serves as a...
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  • Blah
    The Effects of Substrate Concentration Abstract This experiment was done to see if substrate concentration had an effect on rate of reaction. The substrate concentrations done here was based on 0%, 1%, 2%, and 3% H2O2 (Hydrogen Peroxide) using Turnip Peroxidase as the enzyme. This is important...
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  • research paper
    a tube and immediately placed in a spectrophotometer. As hydrogen peroxide is broken down color accumulates, causing the absorbance of 470 nm to increase. The more active the enzyme, the faster will be the change absorbance. Horseradish’s and turnips from which peroxidase is extracted, grow best in...
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  • The Effects of Ph and Salinity on Enzyme Function
    the reaction rate of enzymes? Materials: 13ml of water, .3ml of hydrogen peroxide, guaiacol, clean pipettes, 1.5 ml of turnip peroxidase, 10% salt concentration solution (.6 ml of salt in 6 mL of water), 5% salt concentration solution(.3ml of salt in 6 mL of water), 2% salt concentration solution...
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  • Enzymatic Activity
    Abstract: The objective of this lab was to determine the effects of certain variables on enzymatic activity and to get a quantitative feel for the effectiveness of a certain enzyme, in this case peroxidase obtained from potatoes. In order to do this, we conducted experiments in which the effects...
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  • india a happy and prosperous nation
    antioxidants, such as glutathione, vitamin C, vitamin A, and vitamin E as well as enzymes such as catalase, superoxide dismutase and various peroxidases. Insufficient levels of antioxidants, or inhibition of the antioxidant enzymes, cause oxidative stress and may damage or kill cells. Oxidative...
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  • Food Science
    reversed micellar extraction of enzymes, Chem. Eng. J., 46, B69–74. Duarte-Vázquez, M.A., García-Almendárez, B., Regalado, C., and Whitaker, J.R. (2000) Purification and partial characterization of three turnip (Brassica napus L. var. esculenta D.C.) peroxidases, J. Agric. Food Chem., 48, 1574–1579...
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  • Environmental Variables and Their Effect on Horseradish Peroxidase
    examine the activity or horseradish peroxidase, an enzyme, under a wide range of circumstances and key in on the prime set of conditions of its ideal functionality. The hypothesis proposed is that activity of peroxidase will be at its best under standard rather than extreme settings. In this experiment...
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  • Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato
    Title of Lab: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Hypothesis: Polyphenol Oxidase enzyme activity can be detected by change in colour of solution, Inhibitors prevent the reaction of the enzymes with substrates, the enzyme is relatively specific. Aim: To...
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