process was compared. Acetylsalicylic acid is produced when salicylic acid and acetic anhydride react in the presence of H2SO4. Consequently‚ the said reaction produces acetylsalicylic acid and acetic acid. In part one of the lab‚ titration of a NaOH solution with an indicator and a known concentration into a solution containing
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Hydrogen peroxide. Catalase‚ isolated from potatoes‚ speeds up the breakdown of hydrogen peroxide into oxygen and water. Materials Catalase isolated from potatoes Wax pencil Metric ruler 3 Test Tubes Test tube rack Distilled Water HCl solution NaOH solution pH paper Hydrogen Peroxide
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them to respire for about fifteen minutes. After fifteen minutes remove the organisms from the beakers and return them to their original bowls. Add four drops of phenolphthalein to each beaker. Obtain a burette and add NaOH drop by drop. Mix the contents thoroughly. Record amount of NaOh in millimeters on the table. How much phenolphthalein was required to make the
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| | | Materials: 7 pieces of liver (room temperature) | 1 piece of liver(frozen) | 3 clean test tubes(for each experiment) | Hydrogen Peroxide(2mL) | Hot plate | Beaker | Graduated Cylinder | HCI(2mL) | NaOH(2mL) | Goggles | Lab coats | Stirring rod | Plastic Spoon | Mortar &Pestle | | | | | | | | | | | | | | | | | | |
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Classification for Functional Groups Alcohols Lucas test (Differentiates primary‚ secondary‚ and tertiary alcohols) Reagent: ZnCl2 in conc. HCl Observation: Rate of reaction (tertiary alcohol> secondary alcohol> primary alcohol) Procedure: 2mL Lucas Reagent in test tube+ 3-4 drops of alcohol‚ stopper‚ shake vigorously‚ NOTE time required (less than 10m mins only) to form an emulsion or separate layers. Oxidation (Confirms if alcohol is oxidizable: presence of H in C-OH bond) Reagent:
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transparent‚ low viscosity Test tube felt warm Dark red solid appears as steel wool disappears Solution in test tube turns greenish blue (still transparent‚ low viscosity) 5 Add sodium hydroxide (NaOH) to test tube from change 4 NEXT‚ add hydrochloric acid (HCl) Test tube has greenish blue‚ transparent‚ liquid NaOH – liquid‚ transparent‚ colourless‚ low viscosity‚ odourless HCl – liquid‚ transparent‚ colourless‚
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Experiment II. Separation Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method
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Protein Hydrolysis and Characterization Group 7 Niez‚ Robert Francis‚ *Orbin‚ Alfonso Ricardo* Parro‚ Athena Emmanuelle Peralta‚ Christian Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines • Abstract Hydrolyzed Protein is protein that has been hydrolyzed or broken down into its component amino acids. While there are many means of achieving this‚ two of the most common are prolonged boiling in a strong acid (acid-HVP) or strong base or using an enzyme such
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the calculations in questions 1 and 2. These can be added at the end of this document (see Calculations Section). Complete the yellow shaded regions in the document below. Question #1 Antacid Volume NaOH used in back-titration (mL) Volume NaOH used in back-titration (L) Moles NaOH used in back-titration Maalox 24.1 mL Tums 22.4 mL Mylanta 20.0 mL CVS 19.9 mL Rennies 24.4 mL Based on the values you calculated above‚ which antacid is the strongest / weakest
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20.28% HCI 2 8*10-4 M 2.64 3.09 17.04% HCI 3 3.2*10-5 M 3.38 4.49 24.72% HCI 4 1.3*10-6 M 5.85 5.88 0.51% Bases Concentration of base after dilution Experimental pH Theoretical pH % error NaOH 1 2*10-2 M 11.42 12.31 7.15% NaOH 2 8*10-4 M 9.64 10.91 11.55% NaOH 3 3.2*10-5 M 8.68 9.50 8.63% NaOH 4 1.28*10-6 M 7.98 8.10 1.48% Combined Concentration of acid and base after combination Experimental pH Theoretical pH % error 1 2*10-2 M 6.91 7 1.28% 2 8*10-4 M 6.04 7 13.71% 3 3.2*10-5
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