Preview

Lab Report Enzyme Lab

Good Essays
Open Document
Open Document
743 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Lab Report Enzyme Lab
Abstract: The Enzyme Lab results where when the liver was frozen, its reaction was fast, and when it was hot, it was slow, and the liver that was at room temperature reacted slowly to medium.
Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature, pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions, which would otherwise progress more slowly.(Background Information; pg. 1) pH is a measurement of the acidity or alkalinity (base) of a solution. When the liver got mixed with H2O2 , the first time the chemical reaction was fast, the second time the reaction was slow and the last try was very fast. Temperature is the degree or intensity of heat present in a substance or object. When the temperature of the liver changed from freezing to very hot to room …show more content…
| | |

Materials: 7 pieces of liver (room temperature) | 1 piece of liver(frozen) | 3 clean test tubes(for each experiment) | Hydrogen Peroxide(2mL) | Hot plate | Beaker | Graduated Cylinder | HCI(2mL) | NaOH(2mL) | Goggles | Lab coats | Stirring rod | Plastic Spoon | Mortar &Pestle | | | | | | | | | | | | | | | | | | | Procedure:
Continue Reading
View Writing Issues

You May Also Find These Documents Helpful

  • Good Essays

    Does Tap Water Work The Fastest?
    • 468 Words
    • 2 Pages

    Does Tap Water Work The Fastest?

    But as the enzyme reaches the 40°C mark, the protein will start to denature. As the enzyme reaches around 5°C or lower, the level of enzyme activity will become quicker as the temperatures are more favorable due to its regular cold environment (N.D, PDF). This explains why the iced HCl worked the fastest compared to the other…

    • 468 Words
    • 2 Pages
    Good Essays
    Read More
  • Powerful Essays

    Lab Report: Enzyme Activity
    • 1209 Words
    • 5 Pages

    Lab Report: Enzyme Activity

    (Click on the Save a Copy button on the panel above to save your report)…

    • 1209 Words
    • 5 Pages
    Powerful Essays
    Read More
  • Good Essays

    Enzymes in Living Tissues
    • 416 Words
    • 2 Pages

    Enzymes in Living Tissues

    The purpose of the lab was to analyze the enzymes in living tissues, represented by the pieces of liver. Also, hydrogen peroxide was used to demonstrate these effects.…

    • 416 Words
    • 2 Pages
    Good Essays
    Read More
  • Better Essays

    Salivary Amylase And Phosphorylase As B
    • 2321 Words
    • 9 Pages

    Salivary Amylase And Phosphorylase As B

    The purpose of this lab was to check the time needed for salivary amylase and phosphorylase to produce a negative result and how different concentrations affected those times. Enzymes are biological catalysts that can cause a specific chemical change in any part of the body (Walsh, 2002). Many of the reactions that take place within a cell would normally take place at temperatures substantially higher than those present inside a cell (Alberts et al., 2010). Because of this, these chemical reactions require the assistance of enzymes to catalyze the reaction and allow it reaction to occur quickly (Alberts et al., 2010). The substrate binds with the active site on the enzyme to form a substrate enzyme complex (Di Giuseppe, 2002). The substrate is then transformed into one or more products, which are released from the active site and the enzyme remains unaltered (Di Giuseppe, 2002).…

    • 2321 Words
    • 9 Pages
    Better Essays
    Read More
  • Better Essays

    Biology Open-Ended Investigation
    • 1536 Words
    • 7 Pages

    Biology Open-Ended Investigation

    Introduction: Enzymes are catalysts, because they control the rate of the reaction that helps chemical reactions work properly within living organisms. They are specialised proteins that have a unique shape and chemical composition that creates an active site for connection between the enzyme and substrates. The substrate molecules bind to the active site, inducing a temporary change in the shape of the enzyme known as induced fit. Enzymes catalyse and modify the rate of biomechanical reactions without becoming a part of the product of reaction. Like all proteins, enzymes are made in the ribosomes by linking together amino acids from the cytoplasm. Their action is dependent upon their ability to fit with the molecule reactants; otherwise the reaction will not proceed. For example, study from previous experiments demonstrates that the maltose present will not turn into glucose until it enters the intestine (different catalyst). There are diverse types of enzymes that work best under certain optimum conditions including temperature, pH and substrate concentration. Enzymes can be used more than once and are very important to cell functioning; therefore only small quantities of them are required in the cell. The effect of temperature on enzyme activity is; lower temperatures (0 C) will cause the substrates to move at a significantly slow speed to collide and react with the enzyme therefore very little product will be produced, where as too high of a temperature (100 C) will cause denaturing in the enzyme and very little product will be produced, and lastly room temperature (37 C) will provide the most product of the temperatures being tested. In the presence of starch iodine turns…

    • 1536 Words
    • 7 Pages
    Better Essays
    Read More
  • Satisfactory Essays

    Enzyme Reaction Lab Report
    • 433 Words
    • 2 Pages

    Enzyme Reaction Lab Report

    The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25,30,35,40,45,50,60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture, using a control of previously heated enzyme solution in the reaction.…

    • 433 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Better Essays

    Enzyme Activity Lab Report
    • 2035 Words
    • 9 Pages

    Enzyme Activity Lab Report

    In the following lab factors affecting enzyme activity, temperature, pH, enzyme concentration, substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the rate of reaction will be recorded to see the difference in each factor. In conclusion you will learn whether each factor effects enzyme activity or not.…

    • 2035 Words
    • 9 Pages
    Better Essays
    Read More
  • Satisfactory Essays

    Arginase Lab
    • 497 Words
    • 2 Pages

    Arginase Lab

    As a result, 1mL of enzyme produced 0.6538μmol of urea per minute. It was determined that the liver extract would have produced 4983.47μmoles of urea per minute. For this experiment, the tubes were entirely covered in foil since α-INPP is sensitive to light. The liver extract was also kept on ice to stabilise the enzyme due to the fact that enzymes are very sensitive to temperature. The enzymes perform optimally at 37 degrees Celsius thus the water bath was kept at this temperature. (Nelson and Cox…

    • 497 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Satisfactory Essays

    Enzyme and Ph Lab Report
    • 334 Words
    • 2 Pages

    Enzyme and Ph Lab Report

    The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution had been inserted to test tubes A to D. 3cm³ of hydrogen peroxided was introduced to the tubes then a few drops of detergent had been included to the tubes A to D. We then used test tube rack two with 4 test tubes and labelled them 1 to 4. The 2cm³ of catalase solution had been added to the test tubes labelled 1 to 4. All the test tubes were then placed in the water bath for 5 minutes at 30ºc. The contents inside test tubes 1 to 4 had been added to test tubes A to D. After the enzyme reaction begun we measured the height of the froth in test tubes A to D. The table was prepared and the results had been jotted down. We also repeated the experiment for reliable results.…

    • 334 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Better Essays

    Lab Enzime
    • 969 Words
    • 4 Pages

    Lab Enzime

    Enzymes are made from proteins. They are biological catalysts that speed up the rate of chemical reaction by lowering the activation energy of the reaction. Enzymes are precise and catalyze only detailed reaction. Specificity is an outcome of active site of enzyme that acts on the substrate. Catecholase, which catalyzes a reaction in which catechol, then catechol becomes the product called benzoquinone, which is a reddish-brown color, which make it easier to determine the quantity that has been formed. (Dickey). Moreover, the enzyme activity is persuaded by ph, plus temperature. Change in ph will have effect on hydrogen bonding that can modify the form of protein. Increase in the temperature will spread the reactions rate; nevertheless, if the temperature is unreasonably high the enzymes will denatures. For this experiment we use spectrophometer that measures the color intensity of the solution for particular wavelength. In addition, finding a specific enzyme called Catecholase, as well as the activity of Catecholase in optimal and denaturing environments.…

    • 969 Words
    • 4 Pages
    Better Essays
    Read More
  • Powerful Essays

    Properties of Enzymes and Competitive Inhibitors
    • 1360 Words
    • 6 Pages

    Properties of Enzymes and Competitive Inhibitors

    It was hypothesized that enzyme concentration, temperature, and inhibitors will affect the properties and abilities of the enzyme.…

    • 1360 Words
    • 6 Pages
    Powerful Essays
    Read More
  • Good Essays

    Enzyme Lab Report Essay
    • 514 Words
    • 3 Pages

    Enzyme Lab Report Essay

    Enzymes are catalytic proteins which speed up the rate of reactions. Every enzyme has a specific function – meaning, they can only bind to certain substrates. Because these enzymes are proteins, they can be denatured. Enzymes can be denatured by many factors, such as pH and temperature. This lab was divided into three parts which examined the effects of pH, enzyme concentration and temperature on the rate of which enzymes catalyze.…

    • 514 Words
    • 3 Pages
    Good Essays
    Read More
  • Better Essays

    Effects of Temperature on Catalase
    • 3337 Words
    • 14 Pages

    Effects of Temperature on Catalase

    Time Allowed: 4 weeksDue Dates Task Out first lesson week1, term 1, 2013Risk Ass Due: last Lesson week 2, Term 1, 2013Final: First lesson week 6, Term 1, 2013Procedure: Your task is to investigate the effect of temperature on the activity of enzyme catalase found in beef liver. Use the experimental design and the BioLab Guide provided to assist with your report write up. Safety Considerations:…

    • 3337 Words
    • 14 Pages
    Better Essays
    Read More
  • Better Essays

    Liver And Hydrogen Peroxide Lab Report
    • 2739 Words
    • 11 Pages

    Liver And Hydrogen Peroxide Lab Report

    This investigation could have been improved if more trials were tested, around 4 to 6, this would ensure a better overall average of each temperature and standard deviation to the true value. Adding in 1 or 2 more set temperatures to be tested such as 20oC and 40oC would also give an overall overlook of the reaction rate of enzymes. Where reaction of enzyme activity is at its peak and then when it begins to denature. Another improvement would be to concentrate on one water bath at a time. While testing the reaction rate in one water bath the other may have when left unattended either cooled off or warmed up. Because some of the data was varied compared to other groups, there may of have been an error with the liver itself. While the pure samples of liver were the same surface area, not all pieces were blended from the same section of the…

    • 2739 Words
    • 11 Pages
    Better Essays
    Read More
  • Good Essays

    Effect of Concentration on Enzyme Activity Lab Report
    • 619 Words
    • 3 Pages

    Effect of Concentration on Enzyme Activity Lab Report

    2. Use the small measuring cylinder and teat pipette to measure 4 cm3 of 6% hydrogen peroxide with two drops of detergent into a 100cm3 measuring cylinder and swirl to mix…

    • 619 Words
    • 3 Pages
    Good Essays
    Read More