Properties of Enzymes and Competitive Inhibitors
Index Page Abstract…………………………………………………………………………….….…….3 Introduction……………………………………………………………………….….……..3 Materials and Chemicals used…………………………..……………………..…….…..3 Procedures…………………………………………………………………..…...…...…… 4 Tables……………………………………………………………………………………...5-7 Results……..…………………………………………………………………………...……8 Discussion….………………………………………………..………………………...……8 Conclusion…………………………………………………………………………....…….8 Works Cited ………………………………………………………………………………..9
Properties of Enzymes and Competitive Inhibitors.
Abstract: Properties of enzymes were found in this experiment and some other factors, which affect enzyme activity. Enzymes are catalyst; they catalyze very specific reactions. Results relating to the active site of specific enzymes played a big role while performing this experiment. The purpose of this experiment was to fin how inhibitors affect enzyme’s activity by competing for the active site against substrates.
Introduction: Cells have the ability to perform chemical reactions that at normal temperature outside the body proceed too slowly to support life. Cells are able to perform some reactions rapidly because they possess protein catalyst called enzymes. Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. Each enzyme has a unique globular shape, a small portion of which functions as an active site capable of binding to specific reactants or substrates.
It was hypothesized that enzyme concentration, temperature, and inhibitors will affect the properties and abilities of the enzyme.
Materials:
1Wax Marking Pens
150 ml Beakers
3 400 ml Beaker
1 container of parafilm
1 set of 20 spec tubes
1 regular test tube rack
1 small test tube rack
1 box Kimwipes
Eye Droppers
1 thermometer
2-10ml Graduated Cylinders
1 Spectrophotometer
37 °C waterbath with test tube racks
Solutions:
1 flasks of pH 7 buffered ONPG
1 flask of Lactose 8%
1
Properties of Enzymes and Competitive Inhibitors.
Abstract: Properties of enzymes were found in this experiment and some other factors, which affect enzyme activity. Enzymes are catalyst; they catalyze very specific reactions. Results relating to the active site of specific enzymes played a big role while performing this experiment. The purpose of this experiment was to fin how inhibitors affect enzyme’s activity by competing for the active site against substrates.
Introduction: Cells have the ability to perform chemical reactions that at normal temperature outside the body proceed too slowly to support life. Cells are able to perform some reactions rapidly because they possess protein catalyst called enzymes. Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. Each enzyme has a unique globular shape, a small portion of which functions as an active site capable of binding to specific reactants or substrates.
It was hypothesized that enzyme concentration, temperature, and inhibitors will affect the properties and abilities of the enzyme.
Materials:
1Wax Marking Pens
150 ml Beakers
3 400 ml Beaker
1 container of parafilm
1 set of 20 spec tubes
1 regular test tube rack
1 small test tube rack
1 box Kimwipes
Eye Droppers
1 thermometer
2-10ml Graduated Cylinders
1 Spectrophotometer
37 °C waterbath with test tube racks
Solutions:
1 flasks of pH 7 buffered ONPG
1 flask of Lactose 8%
1