Effect of Concentration on Enzyme Activity Lab Report

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Lab Report
(Effect of concentration on enzyme activity)
Biology
Noor Alawadhi
11- KC

Introduction:
An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives, grows, or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of substrate on the enzyme’s reaction.

Research question:
What does the changes in the amount of substrate on an enzyme’s reaction effect on?

Hypothesis:
I forecast that the more concentrated the hydrogen peroxide is the higher the volume of the foam is and the less concentrated the hydrogen peroxide is the lower the volume of the foam will become.

Variables:
Dependent: Volume of foam
Independent: Hydrogen Peroxide (Substrate) and the Concentrations are (6%, 4%, 2%, 1%, 0.5%) ml. Controlled:
* Time (30 s).
* Volume of Hydrogen Peroxide.
* Constant volume of detergent.
* Size of liver cubes.

Materials:
* 100cm3 measuring cylinder.
* 5 or 10cm3 measuring cylinder.
* 2 teat pipettes.
* Forceps.
* Stop watch.
* Sharp knife or razor blade.
* Solution of hydrogen peroxide (6%, 4%, 2%, 1%, and 0.5%). * 10cm3 detergent.
* Fresh liver.
* 100cm3 of distilled water.

Procedure:
1. Cut 6 cubes of liver approximately 1cm x 1cm x 1cm
2. Use the small measuring cylinder and teat pipette to measure 4 cm3 of 6% hydrogen peroxide with two drops of detergent into a 100cm3 measuring cylinder and swirl to mix Warning: hydrogen peroxide is corrosive, avoid contact with the skin and the eyes and wear safety glasses. Wash off any spills with plenty of water. 3. Using the forceps take one cube of liver and place it in the measuring cylinder. After 30 seconds, record the volume the foam reaches in the cylinder...
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