EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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1. A) I would expect the active site of nylonase to consist mainly of polar amino acids with a few nonpolar amino acids as well because the substrate for nylonase is polar overall‚ but has many nonpolar bonds. What makes me think that the nylonase enzyme is polar is that the substrate that would bind to the active site of nylonase has extreme polarity between carbon and oxygen‚ and between hydrogen and nitrogen due to their differences in electronegativity’s‚ but it still has the nonpolar bonds between
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Osmosis Lab Report The essential focus of the experiment was to acquire data for the mass change resulting from osmosis in order to determine the carbohydrate solution of the carrot cells. The carrots were a vegetable used within the experiment with a carbohydrate solution around .5 M. The hypothesis is if there are carrots in different carbohydrate solutions then there will be a percent change in mass. The carrots have large vacuoles that hold water‚ this allows the mass to increase when the hypertonic
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The milgram experiment. The three people involved were: the one running the experiment‚ the subject of the experiment a volunteer‚ and a person pretending to be a volunteer. These three persons fill three distinct roles: the Experimenter an authoritative role‚ the Teacher a role intended to obey the orders of the Experimenter‚ and the Learner the recipient of stimulus from the Teacher. The subject and the actor both drew slips of paper to determine their roles‚ but unknown to the subject‚ both slips
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UTAR FHSC1134 Inorganic Chemistry Trimester 3 Experiment 1 ________________________________________________________________________ Title: Investigating the Properties of Period 3 Oxides Aim: To examine the oxides of Period 3 elements and describe their structure and bonding. Introduction: Generally‚ there are oxides of metals and non-metals. Metals burn in oxygen to form basic oxides while non-metals form acidic oxides. Structurally‚ they are covalent or ionic compounds. You are to
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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Experiment 14: Preparation of 1-Bromobutane Goal: To prepare 1-Bromobutane by the SN2 reaction from 1-Butanol with Sodium Bromide and Sulfuric Acid. Mechanism: Procedure: 1. Place 27g of NaBr‚ 20mL of n-butyl alcohol‚ and 30mL of water into a 250mL round bottom flask. 2. Put the mixture in an ice-water batch and cool briefly‚ then slowly add 23 mL of conc H2SO4 while stirring with a magnetic stirrer. 3. Place a water-cooled condenser and heat the flask until the mixture boils while
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Experiment D-Pre-lab: Synthesis of a Room Temperature Ionic Liquid (RTIL) Name: Morgan Smith Student #:130635880 Date Submitted: November 7‚ 2014 Lab Section: Friday 2:30pm‚ Section 9 T.A: Matt Halloran 1. Refer to the last page. 2. Arrow pushing mechanism: 3. Refluxing is when the reactants are boiled and the vapor that is produced is cooled. When the vapor is cooled it changes back to its liquid state and returns to the flask. 4. The visual cue that allows you to determine that the
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Calorimetry Experiment Purpose: The objective of this lab is to determine the enthalpy change for NaOH(aq) + HCl(aq) NaCl (aq) + H2O(l) Procedure: Before measuring the enthalpy of acid base neutralization‚ my partner Brian and I determined a calorimeter constant‚ using a homemade polystyrene calorimeter. With the following formula and data: qhot= cm (Tf-Ti) qcold=cm(Tf-Ti) SYSTEM DATA SURROUNDINGS DATA Water cold Mass: 50mL Water hot Mass: 50mL C=4.18 C=4.18 Ti=20 C Ti=31
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the hot water from combining it with the cold water. As discussed in a page titled Heat Capacity2‚ the heat capacity is defined as the amount of heat it takes to raise the temperature of a substance by one degree (in this case C). In the experiment performed in lab‚ the heat capacity of the calorimeters was calculated by subtracting the heat gain from the heat loss and then dividing that by the difference in the temperatures between cold water and the combination of the hot and cold water‚ . The heat
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