ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
Premium Bacteria Microbiology
Isolation and Study of Organelles from Pea Seeds by Differential Centrifugation and Corn to Determine Mitochondrial Activity BIOL 230-01 Cell & Molecular Biology October 13‚ 2014 ABSTRACT Since the discovery of the cell in 1665 by Robert Hooke‚ man has always wondered about the complexity and potential functionality. Using pea seeds‚ which is readily available‚ we will be studying the different parts of the cell by separating it into its component parts by
Premium Organelle Mitochondrion Cell
Isolation Have you ever felt isolated from your surroundings‚ either because of language or culture wise‚ being new in town‚ feeling friendless‚ or simply because you have to study while everyone else is out having fun while you study? Well I have had many experiences like those. There was a time in which I had moved from New Jersey to Tennessee and I was the new kid on the block. Also there was another addition in my life
Premium English-language films Walking My Chemical Romance
Extraction of Caffeine from Lipton® Tea Leaves Nathaniel Camangon*‚ Kaila Bumagat‚ Franz Mari Burgos‚ Remigio Callanta Department of Chemistry‚ College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract This experiment mainly focuses on the extraction of pure caffeine by multiple extraction from commercially acquired tea leaves and determining its purity by melting point determination. A 0.02% yield of pure caffeine was obtained from 10.4978g of tea leaves (4 tea bags).
Premium Solubility Caffeine Tea
BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
Premium Photosynthesis Light Chlorophyll
Isolation of Trimyristin from Nutmeg and preparation of Myristic Acid from Trimyristin by Hydrolysis Introduction Over 40% of medicinal chemicals in the world were developed from isolation of the natural source. These sources were from fungi‚ plants‚ bacteria‚ animals‚ etc. The techniques used to isolate and purify has been by extraction‚ distillation‚ recrystallization‚ and chromatography. In this experiment we will be trying to obtain a pure organic compound from a natural source. Because this
Premium Palm oil
LAB REPORT 4 Isolation of genomic DNA from animal tissues Introduction A rapid expansion of DNA analysis in medical‚ biotechnological and basic research has created the need for simple and efficient commercial methods for isolating genomic DNA. Many different methods and technologies are available for the isolation of genomic DNA. In general‚ all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and finally recovery
Premium DNA Molecular biology Ethanol
Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*‚ Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: September‚ 2010 ____________________________________________________________ _________________________________ Abstract: The experiment was done to separate and analyze the components of chili pepper. Chromatography was used because of its
Free Chromatography Thin layer chromatography
exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in net charge. Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography‚ in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography‚ in
Premium Electric charge Ion Molecule
Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
Premium Amino acid