Introduction In this experiment‚ the essential oil eugenol (the main component of oil of cloves) will be isolated from whole cloves using the technique of simple distillation‚ which is often used to isolate liquid natural products from plants. The mixture of whole cloves and distilled water will then be heated to boiling and the distillate (a eugenol/water mixture) will be collected. The eugenol will then be separated from the water by extraction with pentane. The pentane solutions will then be
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Isolation of Eugenol from Cloves Abstract The experiment conducted‚ isolation of eugenol from cloves‚ is the extraction of the essential oil. To perform this experiment cloves and water were mixed together and heated by a heating mantle in a round-bottom flask. A direct stem distillation took place‚ and the oil product was out aside for a week. After one week‚ the isolation of the eugenol actually takes place. Next the thin-layer chromatographic analysis takes place‚ but the experiment
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Edward Cross Organic Chemistry December 10‚ 2012 Title Steam Distillation of Cloves and Extraction of Eugenol Authors Edward J. Cross* Summary The isolation of Eugenol from Cloves begins with whole cloves and then using a steam distillation apparatus to obtain the cloves oil substance. Then with the mixtures of water and dichloromethane to form two immiscible layers. The layers were dichloromethane substance and aqueous The entire substances that are separated into two individual
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Introduction Eugenol is a naturally occurring chemical that has medical applications such as its use as a natural dental anesthetic. It can be extracted from cloves by using the method of steam distillation. The extracted compound will then be separated from the water using methyl chloride‚ which will then be evaporated using a rotary evaporator leaving only the essential oil‚ Eugenol. IR spectroscopy will be used to determine the structure of the extracted compound. Results and Discussion Four
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Experiment 4: Isolation of Essential Oils from Eugenol Background: Anyone who has walked through a forest or who enjoys the variety of fragrances that flowers have to offer knows that many plants and trees have their own distinctively pleasant odors. These odors are due to the volatile essential oils‚ many of which have been prized commodities since ancient times. If a list of commercially important essential oils was compiled the list would exceed 200 essential oils. Thyme‚ garlic‚ peppermint
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Isolation of Caffeine from Tea (12A) Introduction When a person drinks tea or coffee‚ it is not pure caffeine. It also has other natural substances. The purpose of this lab was to isolate caffeine from tea leaves. The methylene chloride removed nearly pure caffeine from the basic tea solution. Caffeine is a white‚ crystalline‚ bitter alkaloid‚ C8H10N4O2 ‚ usually derived from coffee or tea. Caffeine is used in medicine chiefly as a nervous system stimulant. Caffeine is used by humans to ward off
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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To My parents‚ Mr. Therdsak and Mrs. Aree Jainonthee who always behind every success of my life ACKNOWLEDGEMENT I would like to express my sincere thanks to my thesis advisor‚ Asst. Prof. Dr. Duangporn Pichpol for her invaluable suggestions and encouragement throughout the period of this research. I am most grateful for her teaching and advice on research methodologies and techniques performed in microbiology testing. Her close attention on my working life and my career path are very much appreciated
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Isolation‚ Morphological‚ and Biochemical Characterization. A high-throughput screening of cartenoid-producing microorganisms resulted in 25 astaxanthin-producing bacterial strains from marine water samples that were collected from the Pacific coast of Japan (unpublished results). Among the isolates‚ a novel‚ highly selective astaxanthin-producing marine bacterium‚ labeled strain (N-5)‚ was isolated based on its ability to produce orange pigment on NA plate after 2 days of cultivation at 30 °C. Strain
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Isolation of exosomes from human serum The initial volume of serum for the experiments was 4 mL per sample. The samples were diluted with an equal volume of PBS (AppliChem‚ St. Louis‚ MO) to decrease the viscosity. The diluted serum samples were centrifuged at 2‚000×g for 10 min and 10‚000×g for 30 min at 4 °C to remove dead cells and cell debris. The supernatant was transferred into Ultra-ClearTM tubes (Beckman Coulter‚ Indianapolis‚ IN) and centrifuged at 100‚000×g using a Beckman Optima XL-70
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