Date of experiment: September 3rd‚ 2013 Organic Chemistry II – CHLB330 Name: Symone MoxeyLab Partner: Lynden Cooper Isolation of caffeine from tea leave Abstract:- Introduction:- Caffeine is probably the most commonly used addictive drug. This drug is found in a number of things ingested by people. It belongs to a group of nitrogen bases called purines. Because caffeine possesses these base-like properties‚ it is described as an alkaloid. They usually taste bitter and often are physiologically
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Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
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found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis‚ chlorophyll a occurs in green plants while chlorophyll b occurs in green algae.Carotene and Xanthophyll are carotenoid where it can protect the human eye from being harm
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of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography Substances present in a mixture are allowed to distribute themselves between two phases: the stationary phase (fixed) and the mobile phase. As the mobile phase flows over the stationary phase‚ components of the mixture experience many transfers
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THE STUDY OF CHLOROPHYLL CONTENT IN VARIOUS PLANTS A Project Report Submitted by SANDEEP SETHI In partial fulfillment of the CBSE GRADE XII IN BIOLOGY AT 2015-16 CERTIFICATE This is to certify that Sandeep Sethi of grade XII‚ ST. XAVIER INTERNATIONAL SCHOOL‚ BHUBANESWAR with Registeration Number _______________________ has satisfactorily completed the project in Biology on STUDY OF CHLOROPHYLL CONTENT IN FIVE DIFFERENT SPECIES OF PLANTS‚ in partial fulfillment of the requirements
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Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
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Introduction It is crucial in the field of microbiology to study the morphology‚ biological activity‚ and the behavior of microbes thus the isolation of such microbes from the environment is necessary because it allows scientists to study them in close proximity. Microbial life forms are found everywhere. They are major players in biogeochemical cycling of elements and primary producers in numerous environments. There are also some microbes living inside higher forms of organisms that aid in digestion
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Gas Chromatography Purpose: The purpose of the gas chromatography lab is to find out how different substances interact with the surface of a solid. Chromatography is a separation technique that depends on the relative distribution of the components of a mixture between a mobile phase and a solid stationary phase. Chromatography measures the tendency of a substance to interact with the surface of a solid or to remain in a mobile phase. When doing a chromatography lab the mobile phase has to
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Candy Chromatography Background info: Paper chromatography is a logical technique used to separate works of a solution. Three examples of how we apply this technique to real-life would be: contaneminants in water‚ separation of plant pigmentation‚ and analysis of narcotics. Source: http://www.discoveriesinmedicine.com/Bar-Cod/Chromatography.html#b Purpose: To find out why candies are different colors. * Materials: Candy with a colored coating‚ like Skittles® or M&Ms® (4 different
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GAS CHROMATOGRAPHY EXPERIMENT The purpose of this experiment is for the student: 1) to learn the general theoretical aspects of gas chromatography as a separation method‚ 2) to learn how to operate gas chromatographs specific to COD‚ 3) to become familiar with using the gas chromatograph (GC) to qualitatively identify components of mixtures‚ 4) to be introduced to and to interpret the quantitative data available via gas chromatography‚ 5) to gain insight into how the GC technique is used
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