Name Date Lab: Denisty – Solids and Liquids Part 1: Density Background Information: The density of an object is how much “stuff”‚ or molecules‚ a substance is made of. Density is how tightly packed the molecules are. To calculate the density of a substance we need to know the mass (weight) of the substance and the volume (how much space) of the substance. Mass is determined by weighing an object. Volume of regular shaped objects is calculated by measuring
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Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican‚ Karen Gem B. Ares‚ Ruvie Ann A. Ballester‚ Mark Joseph S. Barcelona‚* Katherine Carmen Isabel G. Calleja‚ Christelle Venus F. Capuno‚ Group 1‚ 2DPH‚ University of Santo Tomas Abstract Lipids are one of the major constituents of foods‚ and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment determined the
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Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas putida A Project done under the guidance of Dr. K. Bharathi Department of Biotechnology. Submitted to the faculty Of Department of Biotechnology National Institute Of Technology‚ Warangal (A.P) Submitted By Febin P. Nalpady‚ Anzal Rahman‚ Shruti Sharma‚ Sindhuja Nandiraju‚ Giraboina
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CD can be used to monitor the binding if s substrate to a protein. The substrate can give a very different CD spectrum when free in the solution relative to when bond in solution. Outside of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography Substances present in
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Experiment 4 – Liquid Phase Chromatography I. Objectives This experiment’s goal is to explore one-dimensional and two-dimensional paper chromatography. II. Schematic Diagram of the Procedure PAPER CHROMATOGRAPHY Wash leaves‚ cut them into smaller pieces; in a mortar macerate them in circular motion Add 8mL ethyl alcohol to extract pigments‚ continue macerating until finely grounded Transfer extract to evaporating dish‚ allow to conc‚ don’t let extract to dry out Concentration
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Experiment 5 Analysis of Analgesic Tablet by High Performance Liquid Chromatography Abstract An unknown sample‚ 529‚ was tested using high performance liquid chromatography to detect the concentrations of acetaminophen‚ aspirin‚ and caffeine respectively. There was found to be 4.03±0.144mg/100mL of acetaminophen‚ 11.5±0.185mg/100mL of aspirin‚ and 4.89±0.185mg/100mL of caffeine. Based on accepted values‚ the maximum daily amounts of each compound are 4000mg of acetaminophen
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EXPERIMENT 2: SUSPENDED SOLIDS 1.0 OBJECTIVE This objective of this experiment is to determine the quantity of suspended solids in polluted water samples. 2.0 INTRODUCTION Water quality is vitally important in our daily life. However‚ due to water pollution‚ the quality of water is questionable for safe usage. In order to determine water quality‚ one of the indicator and measurement that can be used is by suspended solids (SS). Suspended solids is one type of physical water-quality measurement
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COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos‚ Kuia B. Allarde‚ Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment‚ the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to
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Acylation of Ferrocene and Column Chromatography Introduction: The intention of this lab is to analyze the formation of acetylferrocene using column chromatography. The Friedel-Crafts acylation reaction created acetylferrocene and diacetylferrocene‚ using phosphoric acid as a catalyst for the reaction between acetic anhydride and ferrocene (once applying heat). During column chromatography‚ a solution is passed through a filtration system of silica‚ sand‚ and cotton. This creates colored or colorless
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Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart
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