Analysis of Analgesic Tablet by High Performance Liquid Chromatography
Experiment 5
Analysis of Analgesic Tablet by High Performance Liquid Chromatography
Abstract An unknown sample, 529, was tested using high performance liquid chromatography to detect the concentrations of acetaminophen, aspirin, and caffeine respectively. There was found to be 4.03±0.144mg/100mL of acetaminophen, 11.5±0.185mg/100mL of aspirin, and 4.89±0.185mg/100mL of caffeine. Based on accepted values, the maximum daily amounts of each compound are 4000mg of acetaminophen, 520mg of caffeine, and 400mg of aspirin.
Introduction
This experiment was performed to determine the concentrations of acetaminophen, aspirin, and caffeine in the unknown sample 529. High performance liquid chromatography (HPLC) was the technique used. Using HPLC allowed for relatively quick runtime on each sample and highly accurate results. With HPLC, the mobile phase can be a part of dividing the components as opposed to other techniques such as gas chromatography where only the stationary phase is used to separate.
Theory
The purpose of chromatography is the efficiently separate different components in a single sample. The difference in retention time between the two peaks divided by the average base width of the peaks, R, is used as a measure of separation between compounds. The minimum value of R is generally 1.0. The factor K’ is also used for measurement. It can be considered as retention volume in terms of the volume of the component in the column. The higher the capacity factor K’ is, the higher the retention capacity of the column and in turn the longer the retention time for the component. Once the retention value (V₀) has been found it is divided into the net retention volume of the peak (V-V₀) to find k’ = (V-V₀)/V₀. Essentially, small k’ values are good for faster separation and larger k’ values have better resolution. From the view of maximum separation per unit time something between 2 and 6 would be a compromised k’ value. The
Analysis of Analgesic Tablet by High Performance Liquid Chromatography
Abstract An unknown sample, 529, was tested using high performance liquid chromatography to detect the concentrations of acetaminophen, aspirin, and caffeine respectively. There was found to be 4.03±0.144mg/100mL of acetaminophen, 11.5±0.185mg/100mL of aspirin, and 4.89±0.185mg/100mL of caffeine. Based on accepted values, the maximum daily amounts of each compound are 4000mg of acetaminophen, 520mg of caffeine, and 400mg of aspirin.
Introduction
This experiment was performed to determine the concentrations of acetaminophen, aspirin, and caffeine in the unknown sample 529. High performance liquid chromatography (HPLC) was the technique used. Using HPLC allowed for relatively quick runtime on each sample and highly accurate results. With HPLC, the mobile phase can be a part of dividing the components as opposed to other techniques such as gas chromatography where only the stationary phase is used to separate.
Theory
The purpose of chromatography is the efficiently separate different components in a single sample. The difference in retention time between the two peaks divided by the average base width of the peaks, R, is used as a measure of separation between compounds. The minimum value of R is generally 1.0. The factor K’ is also used for measurement. It can be considered as retention volume in terms of the volume of the component in the column. The higher the capacity factor K’ is, the higher the retention capacity of the column and in turn the longer the retention time for the component. Once the retention value (V₀) has been found it is divided into the net retention volume of the peak (V-V₀) to find k’ = (V-V₀)/V₀. Essentially, small k’ values are good for faster separation and larger k’ values have better resolution. From the view of maximum separation per unit time something between 2 and 6 would be a compromised k’ value. The
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