Chromatography
COLUMN AND THIN LAYER CHROMATOGRAPHY
Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino
Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang
Group 1 2C Pharmacy Organic Chemistry Laboratory
ABSTRACT
In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to be separated with respect to the mobile and stationary phases, wherein the stationary phase/s are silica gel and silica gel G, while the mobile phase/s are the extracts on different solvent system.
INTRODUCTION This experiment aims to make the students capable of separating the colored components of siling labuyo and malunggay leaves using column chromatography. Determine the purity of the components using the thin layer chromatography (TLC). Measure the Rf values of the colored components in TLC.
Chromatography is the most orient laboratory technique that deals with separation of homogenous and free of interferences mixtures and identification of compounds. Also, it is essential for analyzing complex compounds. The said method is separated into two phases namely stationary and mobile; it is based on differential partitioning. The mobile phase carries the solutes through the stationary phase. This can be either liquid or gas. The stationary phase is the part of the chromatographic system though which the mobile phase flows. This can be either solid or liquid that may be immobilized or absorbed on a solid. There are various types of chromatography – solid-liquid (column, thin layer), liquid-liquid (paper, high-performance liquid), and gas-liquid (vapor-phase) methods. Results may vary on the speed rate of constituents, which can be measured through the Rf values. Chromatography may be classified as preparative or analytical. Preparative Chromatography resembles
Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino
Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang
Group 1 2C Pharmacy Organic Chemistry Laboratory
ABSTRACT
In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to be separated with respect to the mobile and stationary phases, wherein the stationary phase/s are silica gel and silica gel G, while the mobile phase/s are the extracts on different solvent system.
INTRODUCTION This experiment aims to make the students capable of separating the colored components of siling labuyo and malunggay leaves using column chromatography. Determine the purity of the components using the thin layer chromatography (TLC). Measure the Rf values of the colored components in TLC.
Chromatography is the most orient laboratory technique that deals with separation of homogenous and free of interferences mixtures and identification of compounds. Also, it is essential for analyzing complex compounds. The said method is separated into two phases namely stationary and mobile; it is based on differential partitioning. The mobile phase carries the solutes through the stationary phase. This can be either liquid or gas. The stationary phase is the part of the chromatographic system though which the mobile phase flows. This can be either solid or liquid that may be immobilized or absorbed on a solid. There are various types of chromatography – solid-liquid (column, thin layer), liquid-liquid (paper, high-performance liquid), and gas-liquid (vapor-phase) methods. Results may vary on the speed rate of constituents, which can be measured through the Rf values. Chromatography may be classified as preparative or analytical. Preparative Chromatography resembles
References: [1]Bayquen A. et.al.(2009).Laboratory Manual in Organic Chemistry.Quezon City: C&E Publishing Inc.do.edu/hndbksupport/ochemlabtech.html 2003 [2]Chemistry http://www.wpi.edu/Academics/Depts/Chemistry/Courses/General/tlc.html AUG 31 2011 [3]Chromatography http://en.wikipedia.org/wiki/Chromatography AUG 31 2011 [4]Chromatography http://www.chemguide.co.uk/analysis/chromatography.html AUG 31 2011 [5]TLC (Thin Layer Chromatography) http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html AUG 31 2011