Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas Putida

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Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas putida

A Project done under the guidance of Dr. K. Bharathi
Department of Biotechnology.
Submitted to the faculty
Department of Biotechnology National Institute Of Technology, Warangal (A.P)
Submitted By
Febin P. Nalpady, Anzal Rahman, Shruti Sharma, Sindhuja Nandiraju, Giraboina Kranthi Kumar NATIONAL INSTITUTE OF TECHNOLOGY WARANGAL (A.P)



This is to certify that the project entitled “………………….” carried out by ….., bearing roll no. ….,, final year B.Tech, Biotechnology, during academic year 2010-2011, is a bonafide work submitted to the National Institute of Technology, Warangal in partial fulfillment of the requirements for the requirements for the award of the Degree of Bachelor of Technology.

Guide : Dr. K Bharathi

Dept. of Biotechnology
NIT Warangal


With great pleasure and deep sense of gratitude, we take this opportunity to express our sense of indebtedness to Dr. K Bharathi, our project guide for accepting us under her good self to carry out this project work, and providing us his invaluable guidance and constant encouragement at each and every step throughout the progress of this project. To be sincere it was an inextinguishable treasure of pleasure for us to work under her excellent guidance. I would also like to thank the faculty of our department,Dr. P Sreenivasa Rao, Mr. Onkara Perumal and Mr. K. Narasimhulu. They were a pillar of strength for us and encouraged us to do our best.

Roll no

Table of Contents
1. Abstract
2. Introduction
2.1 Bioremediation
2.2 The conventional techniques of remediation
2.3 Advantages of Bioremediation
2.4 Microbes that are useful for bioremediation

3. Review of literature
3.1 Microbial degradation
3.2 Biodegradation of petroleum hydrocarbons
3.3 Factors affecting Degradation
3.4 Mechanism of Petroleum Hydrocarbon Degradation

4. Brief outline of the project work
5. Materials and Methods
5.1 Preparation Of Nutrient Broth
5.2 Preparation of Nutrient Agar Slants from the Bacterial Strain 5.3 Preparation of SubCultures of Bacterial Strain
5.4 Centrifugation of Crude Oil
5.5 Subculturing Of Petri Plates with oil
5.6 Biodegradation Studies
5.7 Gravimetric Analysis

6. Results
6.1 Growth Analysis of Pseudomonas Putida
6.2 Gravimetric Analysis

7. Discussion
8. References


Oil spills have become a serious problem with the ever-increasing resource exploitation, transportation, storage, and accidental leakage of oil. Several techniques, including physical, chemical, and biological methods, are used to recover spilled oil from the environment. Bioremediation is a promising option for remediation since it is effective and economic in removing oil with less undue environmental damages. However, it is a relatively slow process and the degree of success depends on a number of factors. These factors include the existence of a microbial population capable of degrading the pollutants, the availability of contaminants to the microbial population and the environment factors are type of soil, temperature, pH, the presence of oxygen and nutrients. This project aims...
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