DIFFERENT TYPES OF AGAR Mac Conkey‘s Agar plate MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria‚ except Enterococcus and some species of Staphylococcus)‚ crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at
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instability‚ usually bactericidal. It hinders or kills bacteria. Ceftriaxone sodium is a broad-spectrum bactericidal cephalosporin antibiotic. Ceftriaxone is active in vitro against a wide range of Gram-positive and Gram-negative organisms‚ which include β-lactamase producing strains. Ceftriaxone is indicated in the treatment of the following infections either before the infecting organism has been identified or when known to be caused by bacteria of established sensitivity. Fig. 1.2 Chemical Formula
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Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential
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Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
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certain antibiotic. Understanding the principals of living microbes and identifying my unknown bacteria through numerous biochemical and metabolism tests‚ with the outmost confidence‚ Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the experiments and comparing them to the other six known bacteria: Micrococcus luteus‚ Staphylococcus aureus‚ Staphylococcus epidermidis‚ Alcaligenes faecalis
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colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test | Purpose | Reagents | Observations | Results | Gram stain | To determine the Gram reaction of the bacterium | Crystal violet‚ Iodine‚ Alcohol‚ Sarasin | Pink rod | Gram-negative rods
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unknown bacteria. An unknown labeled as number 3 was given out by the lab instructor. The methods that have been lectured and absorbed pertaining to identifying
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It is very critical in microbiology to determine the effectiveness of a chemical to either inhibit or kill an organism as it can help cure a disease. If the agent kills the bacteria‚ it is called bactericidal. If the agent inhibits the growth of bacteria‚ it is called bateriostatic. (Fundaments of Microbiology Lab Manual) The purpose of this experiment was to examine the effect different disinfectants and antiseptics‚ which are used to decrease the number of microbes‚ on Escherichia coli and Staphylococcus
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Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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inoculation loop being to hot and incinerating the bacteria cells‚ meaning the loop should have had more time in order to properly cool down. The Brain Heart Infusion plate is a complex medium that does not inhibit the microorganisms‚ thus
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