The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme‚ catalase‚ protects the bacteria from the toxic by-products of oxygen metabolism. This enzyme is produced by bacteria that respires using oxygen. The catalase-positive bacteria include strict aerobes. Catalase-negative bacteria may be anaerobes‚ or they may be facultative anaerobes do not respire using oxygen as a terminal electron acceptor. The test reaction is very fast and
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difficile and why is it relevant to us? A.“Clostridium difficile (C. difficile) is a bacterium that may develop due to the prolonged use of antibiotics during healthcare treatments.” 1 B. “Clostridium difficile is an obligate anaerobe or microaerophilic‚ gram-positive‚ spore- forming‚ rod-shaped bacillus.” 2 II. What are the signs and symptoms of Clostridium difficile infection (CDI)? A. C. difficile can cause symptoms ranging from diarrhea to life-threatening inflammation of the colon (pseudomembranous
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The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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(2013) reported a method for isolation of E. coli O157 from meat sample. Twenty five grams of meat sample was blended with 225 ml of sterile modified tryptone soya broth containing vancomycin (40 μg/ml) followed by incubation at 37 °C for 18 h. Enriched culture was plated onto sorbitol MacConkey agar supplemented with cefixime and potassium
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CHAPTER I INTRODUCTION 1. ENTEROCOCCUS FAECALIS AND STAPHYLOCOCCUS AUREUS OUTLINE The Human indigenous micro flora consists of opportunistic pathogens along with other nonpathogenic bacterial strains. Two eloquent members of the category of gram- positive bacteria‚ Enterococcus faecalis and Staphylococcus aureus belong to this category. Being a facultative anaerobe‚ aero tolerant anaerobe respectively‚ both of these organisms inhabited the human body for centuries. Ability to thrive in extreme condition
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The spot inoculation method was followed for antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚
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sample was taken from a door handle to the George Lynn Cross Hall‚ which is touched by hundreds of students entering the building every day. The most important exercises performed include gram staining‚ which
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_____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the colonies formed: Yeasts: Moist‚ creamy bacteria-like‚ opaque‚ or pasty colonies on media. They reproduce by budding. (when they start budding‚ they cause infections) Molds (filamentous fungi): Fluffy cottony‚ woolly or powdery colonies on medium. They reproduce by sporulation. 2. Describe or
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BACTERIA Period: 4 Characteristics: 3 major shapes Cocci Basilli Spirilla 3 major components Mesosomes flagella Plasmids Growing Up: Bacteria can obtain energy through phototrophs(sunlight)‚ lithotrophs(inorganic compounds)‚ and organotrophs(organic compounds) Marriage/Reproduction Binary Fission: The process by which all bacteria reproduce. It results in the separation of a single cell into two. Transformation: genetic alteration
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Bacillus sp.: The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp.‚ on the basis of morphological and biochemical characteristics. Gram’s staining: Gram positive and rod shape. They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution. Visual identification: When bacterial biomass mixed with silver nitrate solution
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