unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative. I also used the microscope to identify
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I was able to come to the conclusion that my unknown is Klebsiella pneumoniae through the use of Bergey’s Manual. After performing a gram stain of my unknown I was able to determine that my unknown was a gram-negative rod. While testing the oxygen requirements my test tube had growth throughout the tube‚ indicating my unknown was a facultative anaerobe‚ which can grow in the presence or absence of oxygen. The most important test was whether my unknown was motile or nonmotile. My test tube only showed
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This semester while we worked through this unit‚ we were trying to figure out why Addie got so sick and how we could look at the juncos as a comparison study. We learned about the relationship that antibiotics and bacteria have by looking at our NetLogo Simulation and Petri Dish Lab. Also‚ we learned about various CDC recommendations for using antibiotics and preventing bacterial illnesses. When we looked at the juncos‚ we were able to learn how common ancestry works within any species. Throughout
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“Fighting Invisible Killers” Scholastic Scope: The Language Arts Magazine January 2014 Edition‚ pages 5-9 Bacteria surround us every day. These little “bugs” are invisible to the eye and most do not harm us. Many are necessary for us to survive‚ like the bacteria in our stomachs and intestines that help us to digest food. But some bacteria are very dangerous to us. Addie Rereich became very sick in May 2011‚ when she was 11 years old. What started as something that her doctors and mother
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Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
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Bacteria are the most numerous type of microorganism found in the rhizosphere of the soil. They produce secondary metabolites which are capable of producing antibiotic which eventually inhibit or kill bacteria. The rhizosphere region of the soil is a highly favorable habitat for the proliferation‚ activity and metabolism of numerous microorganisms. The magnitude of this area depends on the plant and the size of the roots that the plant possesses. Bacteria are among the microorganisms living in the
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Bacterial Contamination April 15‚ 2013 Bacteria Contamination The definition of bacterial contamination is food contamination refers to foods that are spoiled or tainted because they either contain microorganisms‚ such as bacteria or parasites‚ or toxic substances that make them unfit for consumption This is very serious and people should take more precaution‚ food contamination is a serious because it results in foodborne diseases that each year affect an estimated seventy-six million
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Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology Dissecting set
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Then we opened the tubes and using a sterile pipet we put 250 µl of transfer solution in and placed them on ice. Next we removed them from the ice and used a sterile loop to pick up a single colony of bacteria. We put a colony in both tubes and then placed both tubes back on the ice. After that‚ we placed a loopful of plasmid DNA into the positive pGLO. We then incubated the tubes on ice for ten minutes. After the ten minutes were up‚ we placed the tubes
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3.1. Isolation and identification of Bacterial isolates An enrichment culture technique was used for the isolation of bacteria responsible for biodegradation of phorate in soil. Screening of these bacterial species for phorate degradation in liquid cultures in our previous study (Jariyal et al.‚ 2014)‚ resulted in identification of bacterial species B. aerophilus strain Imbl 4.1 ‚ Brevibacterium frigoritolerans strain Imbl 2.1 and Pseudomonas fulva strain Imbl 5.1. However‚ these bacterial species
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