The Comparative Analysis of Simple Staining and Gram Staining Techniques by observing E. Coli and S. Pyogenes under the Compound Light Microscope INTRODUCTION: A German bacteriologist‚ Dr. Theodore von Escherich‚ was the first man in 1885 who discovered the bacterium named Escherichia coli‚ which are gram negative and appears in rod shaped. Most kind of bacteria E. Coli does not cause diseases and some strains indeed are beneficial in helping the process of food breaking down in the intestines
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab sens test. A known Gram positive coccus bacteria will be used alongside an isolated Gram positive unknown bacteria for comparison to aid
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Escherichia coli Abstract Antimicrobial effects of cilantro against the gram positive bacteria S. epidermis and the gram negative bacteria E. coli were looked at. We predicted that cilantro would act as an antibiotic against S. epidermis but not to E. coli because S. epidermis does not contain an outer membrane whereas E coli. does. We predicted that the semi-permeable outer membrane of E. coli would protect the bacteria from any antimicrobial properties of cilantro. A drop of cilantro juice
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Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria‚ it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Apply the primary stain Apply Mordant Apply decolorizing agent Apply the secondary stain Bacteria that lose color
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second culture or a control culture was given to you‚ after failing to get isolated colonies from your original mixed culture and you observed isolated colonies at that point‚ the 1 point is not earned.) Identified Gram Negative bacteria: (2.5 points) Identified Gram Positive bacteria: (2.5 points) Report is written free of grammatical or mechanical errors as determined by the TA. (3 points) Source: Stout‚ V. MIC 302 Report Writing Guidelines. Arizona State University
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Another sample was applied to a glass slide‚ heat fixed and a Gram stain test was performed. From the list of several possible choices for the unknowns‚ about half are eliminated. Table 1 indicates the tests that were performed on the unknown sample‚ all materials required for each test‚ as well as factors determining a positive or negative result. Test Performed Media/Reagents Used Positive Test Observations Negative Test Observations Gram Stain Crystal violet Gram’s iodine 95% Alcohol
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nature. Gram staining is a technique commonly used in the laboratory. It is used to differentiate two large groups of bacteria based on their different cell wall constituents. It distinguishes between Gram positive and Gram negative groups by colouring these cells red or violet. When bacteria are stained by crystal violet‚ Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls which retains the crystal violet while Gram negative bacteria stain
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The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
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The Effects of Antibiotics on Bacterial Growth Biology II 1996 Bacteria are the most common and ancient microorganisms on earth. Most bacteria are microscopic‚ measuring 1 micron in length. However‚ colonies of bacteria grown in a laboratory petri dish can be seen with the unaided eye. There are many divisions and classifications of bacteria that assist in identifying them. The first two types of bacteria are archaebacteria and eubacteria. Both groups have common ancestors dating to more
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