Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat is a form of energy and would increase the reaction and speed
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Name: ______________________________________ Date: ________________________ Student Exploration: Dehydration Synthesis Vocabulary: carbohydrate‚ chemical formula‚ dehydration synthesis‚ disaccharide‚ glucose‚ hydrolysis‚ monosaccharide‚ polysaccharide‚ valence Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. If you exercise on a hot day‚ you need to worry about dehydration. In this context‚ what do you think dehydration means? When you are exercising in the heat you
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Why is water important for living organisms? Water is very important in all living organisms‚ it is vital for many different things. Around 70% of our body weight is due to water. The quantity is high in organs such as lungs and brain and fluids such as blood‚ lymph‚ saliva and secretions by the organs of the digestive system. First of all water is vital in the regulation of body temperature to depending on the temperature mammals will alter the amount they sweat. If they are to warm the amount
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tests that were carried out to help identify them‚ these were: Iodine Test‚ Solubility in Water‚ Benedict’s test‚ Acid Hydrolysis‚ Barfoed test and Diastix test. Aim The aim of the experiment which was carried out was to identify the unknown carbohydrates by subjecting them to a series of biochemical tests. The tests were as follows: Iodine‚ Solubility‚ Benedict’s‚ Acid Hydrolysis‚ Diastix and Barfoed test. Methods There was 1g of each solid carbohydrate contained in bottles labelled A-F‚ 10ml
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sweet smell b. From Acyl Halides Ethanol + H2O + H2SO4 - produced a sweet smell Hydroxamic acid test for the Ester group Ethyl acetate in ether + NH2OH.HCl + KOH heat effervescence + alcoholic HCl + FeCl3 – red colored solution Hydrolysis of acid derivatives H2O + Acetic anhydride 2-immiscible layer formed‚ blue litmus paper turned red‚ test tube becomes warm as the reaction took longer time Pinch of benzamide + NaOH Red litmus paper turned blue III. DISCUSSION INTRODUCTION
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but inhibits its hydrolysis (and thus its transformation into ES-diol ) susceptiblement by more effective adsorption phenomenon. It is observed in this case 89.45% α + β O disappeared for 33.92% of ES-diol product‚ 56% of ES α + β missing. However‚ by amending formate (carbon source and electron) to medium (one obtains E-Sh-Ox-f)‚ the production rate in ES-diol after 14 days of incubation reaches 81.93 % to 93.31% α + β
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Carboxylic Acids and Their Derivatives Ma. Marielle M. Medura Prof. Emma Boncales Chem 23A (TTH 01:00-4:00 p.m) Carboxylic Acids and Their Derivatives I. Introduction Carboxylic acids is an organic compound that contains a carboxylic group(-COOH). Its general formula is R-C=OOH with R referring to the rest of the molecule such as H and C. They are directly attached to a carbonyl group and the interaction between them affects the reactions of each. The polarity of the O-H bond
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Kinetics of an SN1 reaction: the effect of solvent on rate Object The purpose of this experiment is to determine the rate of hydrolysis in acetone/water (50/50 v/v and 60/40 v/v). Background and Theory An SN1 reaction of tert-butyl chloride takes place in two steps. First‚ the Alkyl Halide will leave the molecule. In this step the bond is breaking‚ which takes a longer amount of time‚ so it will determine the rate of the reaction. As a result‚ it forms a tertiary carbocation‚ since this
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A-2Time Blue Color Fades | A-3Time for Starch Hydrolysis | 1 | 1 | 9:07 am | 9:42 am | 35 minutes | 2 | 5 | 9:07 am | 9:37 am | 30 minutes | 3 | 10 | 9:08 am | 9:33 am | 25 minutes | 4 | 15 | 9:08 am | 9:28 am | 20 minutes | 5 | 30 | 9:09 am | 9: 24 am | 15 minutes | Figure 1: Enzyme Activity (Time for Starch Hydrolysis) vs. Enzyme Concentration B. Effect of pH pH | B-1Starting Time | Time Blue Color Fades | B-2Time for starch Hydrolysis | 3 | 9:17 am | 9:47 am | 30 minutes |
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though some drug metabolites are pharmacologically in active status. Inactive substance which has a metabolite that is active is referred to as a prodrug. Drugs are metabolized by the following processes; reduction‚ oxidation‚ conjugation‚ hydration‚ hydrolysis‚ condensation and isomerization. All these processes make the excretion of drugs easier from the body like that of a human being. For metabolism to occur‚ enzymes must be present. Enzymes are present in body tissues but they are concentrated in
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