Exploratory Response Essay
The exploratory phase (Exp No. 1) has highlighted bio-reduction ferrihydrite by Shewanella oneidensis MR-1, which occurs only in the presence of formate (carbon source and electron). The Exp # 2 showed that the presence of the bacterium and iron oxide in the reaction medium (E-Sh-Ox) promotes the disappearance of endosulfan but inhibits its hydrolysis (and thus its transformation into ES-diol ) susceptiblement by more effective adsorption phenomenon. It is observed in this case 89.45% α + β O disappeared for 33.92% of ES-diol product, 56% of ES α + β missing. However, by amending formate (carbon source and electron) to medium (one obtains E-Sh-Ox-f), the production rate in ES-diol after 14 days of incubation reaches 81.93 % to 93.31% α + β
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The degradation process in this case out put by hydrolysis could be biological or abiotic under the influence of Fe (II) product. Fe (II) produced in the E-Sh-Ox-f system could induce degradation of α + β ES, or in another case is adsorbed on ferrihydrite and the complex formed may degrade the α ES + β. This hypothesis can be verified by an experiment for E-Fe modalities (II) -f, E-Fe (II) -Ox-f and Ef in order to observe the degradation rates depending on the presence Fe (II) and Fe (II) complex -Ox. The missing part O α + β could be transformed into another compound or then the ES-product diol to be converted in turn into ES-ether, ES-hydroxy-ether or other product. This could be verified by a UHPLC analysis coupled with mass spectroscopy (UHPLC-MS). It would be interesting to determine the SE of the adsorption kinetics of α + β and ES-diol by performing sorption isotherms on ferrihydrite and the reactor walls. We could do an analysis on the particles in the medium after bio-reduction to see if there would be a new mineral formed. In the case where there is forming a new mineral, one would carry out an analysis. Once identified mineral, it could also perform experiments in establishing E-f-new mineral terms, E-Fe (II) -New mineral-f, E-Fe (II) -New mineral-Ox-f and Ef to evaluate
The degradation process in this case out put by hydrolysis could be biological or abiotic under the influence of Fe (II) product. Fe (II) produced in the E-Sh-Ox-f system could induce degradation of α + β ES, or in another case is adsorbed on ferrihydrite and the complex formed may degrade the α ES + β. This hypothesis can be verified by an experiment for E-Fe modalities (II) -f, E-Fe (II) -Ox-f and Ef in order to observe the degradation rates depending on the presence Fe (II) and Fe (II) complex -Ox. The missing part O α + β could be transformed into another compound or then the ES-product diol to be converted in turn into ES-ether, ES-hydroxy-ether or other product. This could be verified by a UHPLC analysis coupled with mass spectroscopy (UHPLC-MS). It would be interesting to determine the SE of the adsorption kinetics of α + β and ES-diol by performing sorption isotherms on ferrihydrite and the reactor walls. We could do an analysis on the particles in the medium after bio-reduction to see if there would be a new mineral formed. In the case where there is forming a new mineral, one would carry out an analysis. Once identified mineral, it could also perform experiments in establishing E-f-new mineral terms, E-Fe (II) -New mineral-f, E-Fe (II) -New mineral-Ox-f and Ef to evaluate