Tan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube‚ spatula‚ glass rod‚ dropper‚ beaker‚ test tube rack‚ skewer. Materials : - 1 egg‚ meat tenderizer‚ salt‚ water ‚ soap‚ isopropyl alcohol 91%‚ pineapple juice. Variables : Manipulated Variable : Responding
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TAMARINDUS Linnaeus pp. 434-436‚ Manila * Srivastava‚ P. & Malviya‚ R. (2011) Indian Journal of Natural Products and Resources Vol. 2 (1) Sources of pectin‚ extraction and its applications in pharmaceutical industry. pp. 10-18 * May‚ C. D. Handbook of Hydrocolloids pp. 169-188 * Huang‚ J. M. G. Improved method for the extraction of Pectin‚ PO Box 4200 Highstown‚ New Jersey 08520 Acknowledgement I would like to thank God‚ for His guidance throughout this investigatory project. To my
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DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed with 15 g of NaCl and 900 ml of water. Procedures Used in Experiment Once all materials were gathered by group members‚ each
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Objectives 1. To separate a mixture of an acid and a neutral compound into its component by extraction. 2. To determine the melting point and the yield of the benzoic acid and the unknown sample. 3. To determine the unknown sample whether is is Trimethylmethanol or 1‚2‚4‚5-Tetrachlorobenzene. Introduction Extraction is a process of transferring a solute from one solvent to another. It is usually used to separate one or more components from a mixture. Diethyl ether‚ dichloromethane
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Anza Thomas 10/24/2013 CHE 261 (KC) Dr . McAtee Lab : Extraction of Caffiene Purpose: the purpose of this experiment is to extract caffeine from tea using solvent extraction techinques. Reference: Williamson‚ K.; Minard‚ R.; Masters‚ K. Macroscale and Microscale Organic Experiments‚ 2007‚ p. 137 to 171. Procedure: In a 50 mL beaker place 15 mL of water‚ 2.0059 g of sodium carbonate‚ and a wooden boiling stick. Bring the water to a boil on the ceramic heating plate. Remove the beaker
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Experiment title: Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page
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EXTRACTION OF INVERTASE FROM YEAST AND EFFECT OF pH ON ENZYMATIC ACTIVITY Jenelle C. Faustino‚ John Gambit B. Garcia‚ Fatima S. Jusay‚ Oliver Alexander B. Lao and Eunice L. Licudine Group 4 2 E Medical Technology Biochemistry Laboratory ABSTRACT Enzymes are substances that are produced by living organisms and act as catalysts in order to speed up or chance a chemical reaction without changing itself at the end of the reaction. Invertase was extracted first from baker’s yeast. Determination
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What is a Tooth Extraction? A tooth extraction is the process of removing a tooth from the mouth. The procedure may need to be done for a number of reasons. Sometimes before getting braces‚ a tooth needs to be removed in order for the teeth to have enough room to move into their proper location and order for a straight smile. In other cases‚ a tooth may need to be removed because it has decayed or has become loose due to gum disease. Removing it is the first step in stopping the pain and infection
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Kadijat‚ and Kharla Ms. Bulahan Writer: Djina Jan-18-15 Lab #6 - DNA Extraction lab Introduction: DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Cytosine with Guanine. S ince DNA is the blueprint for life‚ every living thing contains DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and ma
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It takes a period of 20 years for the Prunus Africana to grow to 30 meters or more. That is one reason there are only a limited number of Prunus Africana found in Kenya‚ Uganda‚ and the Democratic Republic of Congo. The extraction of pulverized‚ dark bark of Prunus Africana is the reason for high demand on these trees for production of drugs for prostate issues. The Prunus Africana is currently being harvested out of eastern highlands of Kenya in the Aberdares Mountains
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