Isolation of Acetyleugenol (Clove Oil) from Cloves Purpose In this experiment‚ you will obtain the essential oil from cloves by steam distillation. You will then separate a component (acetyleugenol) from the oil by liquid-liquid extraction. Finally‚ you will isolate the acetyleugenol by using a rotovap for reduced pressure distillation‚ which will remove the organic solvent (dichloromethane). Background Whole cloves contain between 14% and 20% by weight of essential oils‚ but
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Introduction Extraction involves dissolving a compound or compounds either from a solid into a solvent or from a solution into another solvent. Extraction is a method used to purify a substance by removing the impurities that was added to it. A solvent- solvent extraction is a type of extraction that is most commonly done by using two substances that are typically insoluble with each other. An acid-base extraction is a solvent-solvent extraction‚ in almost every case one of the solvents is water
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Extraction of DNA from Cheek Cells Gene Smith February 29th 2013 INTRODUCTION DNA‚ deoxyribonucleic acid‚ is the genetic material of every living organism and is found in the nucleus of eukaryotic cells. DNA is often called the ‘blueprint for life’ because it contains the necessary information to carry out all the living processes of the cell (1).The purpose of this lab was to extract DNA from human cheek cells. The isolated DNA could be used inmapping or sequencing‚ PCR‚ crime scene investigation
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Extraction of Caffeine from Tea Leaves Marc Tugaoen‚ Kristine Vanzuela‚ Rafael Villanueva‚ Justeen Wong Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract This experiment has been divided into 4 set-ups‚ first was the solid-liquid extraction‚ next was the liquid-liquid extraction‚ then the sublimation and last was the melting point determination. The solid-liquid and liquid-liquid extraction were both done during the first meeting‚ the DCM layer was filtered and
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A. Title – Acid-Base Extraction with Separatory Funnel B. Introduction – The main objective of the experiment was the extract an acid‚ a base‚ and a neutral compound from a 2:1:1 compound of benzoic acid‚ p-nitroaniline‚ and azobenzene. In theory‚ an extraction technique based on phase distribution should allow two immiscible solvents to separate with a portion of the solute distributed into each. Using a separatory funnel‚ acids were extracted with bases (NaOH)‚ and bases were extracted
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Purpose This experiment deals with separating substances. The objective was to become familiar with the methods of separating substances from one another using decantation‚ extraction‚ sublimation techniques. Brief Introduction This experiment was set to teach research students different methods of separating substances from one another. Materials that are not uniform in composition are set to be impure or heterogeneous and are called mixtures. The separation of the components of mixtures is
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Extraction of Caffeine from Tea Leaves Santos‚ Raphael‚ Samson‚ Nonia Carla‚ *Tabora‚ Brylle‚ Tan‚ Kate Michal Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Tea leaves are natural products that are harvested from agriculture. Caffeine‚ on the other hand‚ is a stimulant that has been shown to speed up the time of reaction‚ increase alertness‚ and improve one’s concentration. What we did in this experiment was to extract the alkaloid caffeine content
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Preparation of SKEO The extraction of S. khuzistanica was performed by hydro distillation and clevenger apparatus for 5 hours. Once extraction was completed‚ the resulted essential oil was dehydrated using sodium sulfate (Merck). The essential oil was stored at temperature of 4ºC until using (12). In order to provide different concentrations of the extract‚ Dimethyl sulfoxide (DMSO) was used. Gas chromatography/mass spectrometry analysis of essential oil Isolation and measurement of the sample
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As the assistant to Michael Brune‚ the executive director of the Sierra Club‚ I am in full support of The Exploratory Fracking and Extraction act. I would like to express concerns associated with hydraulic fracturing‚ or fracking‚ on behalf of the Sierra Club. Critical problems that are commonly brought to attention are: contamination of water supplies‚ seismic activity caused by fracking‚ and it worsens the current condition of the climate. Fracking wells inject water‚ sand‚ and chemicals deep
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microfuge tube. Place this tube directly into liquid N2 to freeze plant tissue. 2) Remove tubes from the liquid N2 and quickly grind the plant tissue with a pestle. Add 300 L of Methanol 1%HCl and continue to grind the plant tissue. Allow extraction to occur overnight in a dark refrigerator. 3) Add 200 L Milli-Q H20 to each tube. Next‚ go to the fume hood and add 500 L of chloroform to each tube. Spin the tubes in a centrifuge set at the highest rpm for 2-5 minutes. 4) Return to
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