DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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Emmanuel Ilodi‚ Ogemdi Okafor‚ etc) for going me a conducive atmosphere to carry out this research I say‚ may God bless and keep you all. Amen. ABSTRACT The extraction of pectin from orange peels has been studied at different sample to solvent ratios‚ different PHs and with different extraction techniques using water. The amount of pectin extracted reduces as the PH increased. This research work is been carried out in order to determine the process condition that will result
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Lab 5 Acid/Base Extractions Introduction The purpose of this experiment is to separate either the organic base (amine) or organic acid (carboxylic acid) from a mixture that contains inorganic impurities (salt) by performing a liquid-liquid extraction and then taking a melting point. Key Experimental Details and Observations Our starting material‚ Compound B‚ was a fine white powder and weighed 0.535g. The final product was a shiny white sheet that resembled acrylic paint and weighed 0.109g
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Extraction of caffeine from Lipton tea leaves Maria Gianna Beatrice L. Cancio*‚ Joe Mari Isabella B. Caringal‚ Rowena A. Chiang‚ Patricia Deanne del Valle Department of Biological Sciences‚ College of Science‚ University of Santo Tomas‚ España‚ Manila‚ Philippines Abstract Caffeine was extracted from Lipton tea leaves by multiple extraction techniques namely solid to liquid extraction and liquid to liquid extraction; the purification by sublimation and melting point determination. Three
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Brandon Schmetterer 3-13-15 Biology labs DNA Extraction Lab DNA is extracted from humans for genetic testing‚ for body identification‚ and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next‚ the cells must be burst open in order to release DNA. Third‚ DNA is separated from protein and debris. Lastly‚ the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis
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The Amplification DNA Extraction from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method
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A REPORT ON THE OVER-EXTRACTION OF GROUNDWATER IN QUEENSLAND Prepared for: THE IDEAS SUMMIT ON THE ENVIRONMENT Due: 22 May 2013 Submitted: 22 May 2013 Prepared By: Katie Gray Executive Summary The purpose of this report is to focus on an aspect of environmental economics‚ namely over-extraction of groundwater in Queensland and provide and evaluate a possible solution for this issue. Groundwater is a valuable resource which is not being protected in Queensland. Its preservation
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EXTRACTION OF CAFFEINE FROM TEA LEAVES ABSTRACT Caffeine extraction from the commercial tea leaves (Lipton Yellow Label Tea) that was done is multiple extraction. 4 tea bags were used in the experiment; tea leaves were weighed having 8.5333 grams. The leaves were boiled in a beaker with 150ml of water; the aqueous tea extract was transferred in a seperatory funnel mixed with DCM (20ml)‚ done thrice. The DCM layer was washed with 20ml 6M NaOH in the seperatory funnel; the organic layer
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Extraction and Evaporation: Separating the Components of "Panacetin" Submitted- July 17‚ 2012 Introduction The purpose of this lab is to investigate the composition of a compound suspected to be Panacetin‚ a type of pain-killer. Panacetin is typically made up of sucrose‚ aspirin‚ and acetaminophen‚ but the third component in this experiment is unknown. The unknown component is suspected to be a chemical relative of acetaminophen‚ either acetanilide or phenacetin. Using techniques such as
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nucleus of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants in this experiment include‚ the shape and size of the coffee filter‚ the amount of extraction buffer used‚ and the amount of isopropyl
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