Extraction of Caffeine from Tea Leaves
Extraction of caffeine from Lipton tea leaves
Maria Gianna Beatrice L. Cancio*, Joe Mari Isabella B. Caringal, Rowena A. Chiang, Patricia Deanne del Valle
Department of Biological Sciences, College of Science, University of Santo Tomas, España, Manila, Philippines
Abstract
Caffeine was extracted from Lipton tea leaves by multiple extraction techniques namely solid to liquid extraction and liquid to liquid extraction; the purification by sublimation and melting point determination. Three teabags with weight 6.2674g were boiled with water and then extracted with 60 mL DCM; extract was dehydrated with anhydride Na2SO4 then was collected by decanting to an evaporating dish where it was evaporated to dryness. Crude caffeine with a weight of 0.0446g gave out a 0.7116% yield; after sublimation, the sublimate weigh 0 .0009g with a percentage yield of 0.0144%. Melting point determination was the last step where the sublimate was compared to a standard caffeine, where both gave out the temperature range of 228 ̊C – 230 ̊C.
Introduction
Caffeine is a bitter substance found in coffee, tea, soft drinks, chocolate, kola nuts and certain medicines. It has many effects on the body’s metabolism, including stimulating the central nervous system. This can make one more alert and give a boost of energy. [1] Most people have high tolerance for caffeine and this can be manifested with the cups of coffee one can consume in a day. However, too much caffeine intake can make one restless, anxious, uneasy, and irritable. It may also keep one from sleeping well and cause headaches, abnormal heart rhythms, or other problems. Age and body size can make a difference in effect. A child or a smaller person may feel caffeine’s effects more strongly than an adult or a heavier, taller person. [2] Caffeine in tea leaves comprises about 5% of its weight. Extraction is a very common laboratory procedure used when isolating or purifying a product. The principle of extraction
Maria Gianna Beatrice L. Cancio*, Joe Mari Isabella B. Caringal, Rowena A. Chiang, Patricia Deanne del Valle
Department of Biological Sciences, College of Science, University of Santo Tomas, España, Manila, Philippines
Abstract
Caffeine was extracted from Lipton tea leaves by multiple extraction techniques namely solid to liquid extraction and liquid to liquid extraction; the purification by sublimation and melting point determination. Three teabags with weight 6.2674g were boiled with water and then extracted with 60 mL DCM; extract was dehydrated with anhydride Na2SO4 then was collected by decanting to an evaporating dish where it was evaporated to dryness. Crude caffeine with a weight of 0.0446g gave out a 0.7116% yield; after sublimation, the sublimate weigh 0 .0009g with a percentage yield of 0.0144%. Melting point determination was the last step where the sublimate was compared to a standard caffeine, where both gave out the temperature range of 228 ̊C – 230 ̊C.
Introduction
Caffeine is a bitter substance found in coffee, tea, soft drinks, chocolate, kola nuts and certain medicines. It has many effects on the body’s metabolism, including stimulating the central nervous system. This can make one more alert and give a boost of energy. [1] Most people have high tolerance for caffeine and this can be manifested with the cups of coffee one can consume in a day. However, too much caffeine intake can make one restless, anxious, uneasy, and irritable. It may also keep one from sleeping well and cause headaches, abnormal heart rhythms, or other problems. Age and body size can make a difference in effect. A child or a smaller person may feel caffeine’s effects more strongly than an adult or a heavier, taller person. [2] Caffeine in tea leaves comprises about 5% of its weight. Extraction is a very common laboratory procedure used when isolating or purifying a product. The principle of extraction
References: [1] retrieved from http://www.nlm.nih.gov/medlineplus/caffeine.html [2] retrieved from www.nlm.nih.gov/medlineplus/druginfo/caffeinesystemic202105.html [3] retrieved from http://www.gunt.de/download/extraction_english.pdf [4] retrieved from http://www.polaris.nova.edu/~shanbhag/chemistry/oc1labs/caffeine.pdf [5] retrieved from http://chem-courses.ucsd.edu/CoursePages/Uglabs/143A_Weizman/expt_3N.pdf