Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA.
To be able to observe white springy substances after mixing with enzyme and alcohol.
Apparatus : -Test tube, spatula, glass rod, dropper, beaker, test tube rack, skewer. Materials : - 1 egg, meat tenderizer, salt, water , soap, isopropyl alcohol 91%, pineapple juice.
Manipulated Variable : Responding Variable : Constant Variable : The different type of enzyme used. Identify the white springy substance as the DNA. The amount of egg yolk in test tube, the drops of enzymes.
Procedure : 1. The egg is cracked open and gently separate the yolk from the white into a small bowl. 2. Then add a spoonful of salt and a few drops of water to the yolk. 3. Use a glass rod and mix the mixture. 4. Take a spoonful of dish soap and mix into the mixture for 5 minutes. 5. The 3 test tubes will be then filled with small amount of yolk mixture using a dropper. 6. A pinch of meat tenderizer will be added into each test tube. 7. The test tube is then added with isopropyl alcohol carefully by using a dropper. 8. Observe and record the results, repeat step 1-6 with another 3 test tube by using pineapple juice as the enzyme.
Figure 1.1 : The arrow shows the white springy
substance as DNA. Visibility is low.
Figure 1.2 : The arrow shows a visible image of
DNA ﬂoats on the surface of alcohol.
After adding 3 drops of isopropyl alcohol and meat tenderizer in the egg yolk solution, after a few minutes you are able to observe a little DNA white springy substance. Once DNA is visible it rose into the clear alcohol that also floats on water. On the test 3 and test 4 , we added 5 drops of isopropyl alcohol into both 3 small test tubes and to investigate the differences of enzyme affecting the visibility of DNA.
Figure 1.3 : Shows the white springy