Procedures for Part A: For Activity A‚ we first tested enzyme activity. First‚ we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled
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Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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immersion lens while observing prepared bacterial slides and also to prepare slides for observable bacteria culture from yogart. Procedure: Exercise 1: Using the Microscope - Viewing Prepared Slides I started out by reading and reviewed the science lab safety reinforcement Agreement and the instruction to oil immersion lens. After setting up my lab station I view the six prepared slides with 10x and 40x objctives lens. In each slide the differences was magnification and the 40x lans gave a clearer
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Lab #1 1. List the five (5) steps of the Hacking process. Reconnaissance‚ Scanning‚ Gaining Access‚ Maintaining Access‚ Covering Tracks 2. In order to exploit or attack the targeted systems‚ what can you do as an initial first step to collect as much information as possible about the targets prior to devising an attack and penetration test plan? The first step would be the reconnaissance or footprinting step of the hacking process. 3. What applications and tools can be used to perform
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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Using different sucrose concentrations Introduction The purpose of this experiment was to estimate Osmolality of plants cells i.e. Potato and celery by converting the observed change of weight in different sucrose concentrations. The hypothesis assumes that the solute concentration of the plant samples would be indirectly proportional to the weight; there would be decrease in weight as the concentration increases. The independent variable in this experiment would be the change in weight
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Experiment 1: Basic Laboratory Techniques Briana S. Golar Laboratory Partners: Dayreal Brown Valerie Marcellus Andrew Thompson January 16‚ 2013 Conclusion Introduction: The intent of the experiment is to sucessfully understand that chemistry is an experimental science which is dependent upon certain observation and the use of good laboratory techniques. The experimenter should become familiar with basic operations necessary
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Structure: Enzymes are globular proteins that act as catalysts‚ they have a specific 3D shape that is the result of their amino acid sequence. There is a specific region of the enzyme that is functional‚ this is called the active site. The active site is made up of a small number of amino acids and forms a small depression within the larger enzyme molecule. Moreover‚ the molecule that the enzyme acts upon (substrate) fits precisely into the depression and forms an enzyme-substrate complex. The substrate
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Attack and Penetration Plan Table of contents 1 Scope 2 Goals and Objectives 2 Tasks 2 Reporting 2 Schedule 2 Unanswered Questions 2 Authorized Letter 2 SCOPE Located on ASA_Instructor‚ the e-commerce Web Application server is acting as an external point-of-entry into the network: Ubuntu Linux 10.04 LTS Server (TargetUbuntu01) Apache Web Server running the e-commerce Web application server Credit card transaction
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The Effects of ECB on Corn Crop Yield Abstract In this experiment we explore the infestation of ECB (European Corn Borer) on different types of corn and how it effects the corn crop yield. The corn is grown in a controlled environment with the only changing variables being the type of corn and the concentrations of the infestation of the ECB. Varying levels of infestation are tested on a corn crop throughout a 140 day growing season‚ at the end of the season the crops are then measured for
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