Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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Experiment 1: Coding In this experiment‚ you will model the effects of mutations on the genetic code. Some mutations cause no structural or functional change to proteins while others can have devastating affects on an organism. Materials Red Beads Blue Beads Yellow Beads Green Beads Procedure: 1. Using the red‚ blue‚ yellow and green beads‚ devise and lay out a three color code for each of the following letters (codon). For example Z = green : red : green. In the spaces below the letter‚ record
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DIFFERENCES BETWEEN PLASMID AND CHROMOSOMAL DNA IN BACTERIA. Eukaryotes have two or more chromosomes‚ prokaryotes such as bacteria possess a single chromosome composed of double-stranded DNA in a loop. DNA is located in the nucleoid of the cell and is not associated with protein A plasmid is an extra-chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently of the chromosomal DNA. Plasmids usually occur naturally in bacteria. A chromosome
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Chapter 13—Chromosomal Rearrangements and Changes in Chromosome Number Reshape Eukaryote Genomes Fill in the Blank |1. |Events that reshape genomes by reorganizing the DNA sequences within one or more chromosomes are known as ____________________. | |Ans: |rearrangements | | |Difficulty: 2
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Chapter 1 DNA‚ RNA‚ Historical Experiments & Structures Frederick Griffith Experiments with Streptococcus pneumoniae S. pneumoniae comes in two strains‚ smooth and rough strains S Smooth : Secrete a polysaccharide capsule; Protects bacterium from immune system of hosts; Produce smooth colonies on solid media. R Rough : Unable to form capsule; Produce colonies with a rough appearance. Griffith conducted experiments in 1928 using two strains of S. pneumoniae: type IIIS and type IIR : 1. Inject
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Balao‚ Mike Paul C. MT1311 Chromosomal aberrations are abnormalities in the structure or number of chromosomes and are often responsible for genetic disorders. For more than a century‚ scientists have been fascinated by the study of human chromosomes. It was not until 1956‚ however‚ that it was determined that the actual diploid number of chromosomes in a human cell was forty-six (22 pairs of autosomes and two sex chromosomes make up the human genome). In 1959 two discoveries opened a new era
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Chromosomal Disorders You will be assigned a random chromosomal disorder. You will research the disorder and answer the questions below. You should include a sketch or picture of the chromosome affected (where the gene is located) or the show the karyotype of the disorder and how it compares to a “normal” karyotype. 1. When was the syndrome first discovered and by who? J. C. P. Williams first discovered it at the year 1961 2. The human chromosomes are numbered. If applicable‚ identify
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Group No. Name: | Date Performed: | Course & Section: | Date Submitted: | Program & Year: | Professor: | Experiment 1 GASLAWS A. Combined Gas Law Temperature of Boiling Water in Kelvin (T1) | | Temperature of Cold Water in Kelvin (T2) | | Atmospheric Pressure in mm Hg (P1) | | Vapor Pressure of Water at T2 in mm Hg | | Final Pressure of Air in mm Hg (P2) | | Volume of Water Collected in the Flask in mL (Vwater) | | Initial Volume of Dry Air in mL (V1) |
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Biotechnology‚ Mapua Institute of Technology ABSTRACT In this experiment‚ solubility class of various organic compounds are to be determined. The main objective of the experiment is to identify an unknown compound through the use of preliminary tests such as examination of physical state‚ color‚ odor‚ and ignition properties. Also‚ solubility tests were used to further examine an unknown compound’s solubility class. In this experiment‚ the apparatus used are micro test tubes and droppers for mixing
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EXPERIMENT 1: THE HEAT CAPACITY RATIO FRANK KAYEYE 100886802 Purpose: The purpose of this experiment was to calculate the heat capacity ratio γ for three gases find in three different classes. The three different gases and their classes are; helium which is classified as a monatomic gas‚ nitrogen which
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