lab 1
Laboratory Report: Observing Bacteria
Purpose: Is to gain knowledge on how to use a microscope and oil immersion lens while observing prepared bacterial slides and also to prepare slides for observable bacteria culture from yogart.
Procedure:
Exercise 1: Using the Microscope - Viewing Prepared Slides I started out by reading and reviewed the science lab safety reinforcement Agreement and the instruction to oil immersion lens. After setting up my lab station I view the six prepared slides with 10x and 40x objctives lens. In each slide the differences was magnification and the 40x lans gave a clearer look of the specimen. The observation of slide e was the mirror image when viewed throughthe micoscope. After observations of the six prepared slides. Then I took out oil immersion len. I observed what great amount of magnification and clarity. the slides was highly focused and easy the view individual cells.
Exercise 2: Observing Bacteria Cultures in Yogurt I prepared a teaspoon of yogurt in a cup and placed it inside my closet for 12 to24 hours. Then I viewed the prepared yogurt slide.
Questions:
A. List the following parts of the microscope and describe the function of each:
A: Eye Piece
B: Main Tube
C: Objective Lens
D: Nose piece
E: Stage
F: Removable filter
G: Light Source or Mirror
H: Course Adjustment I: Fine Adjustment Knob
J: Arm
K: Stage knobs
L: Base
B. Define the following microscopy terms:
a. Focus: To move the specimen closer or furthur awy from the objective lens to render a sharper image.
b. Resolution: Is the ability of the lens system to show fine details of the object being observed.
c. Contrast: (Color) Degree of lightness/darkness part of something. Can be flipped depending on the color of the specimen.
C. Describe your observations from fresh yogurt slide you prepared in
Purpose: Is to gain knowledge on how to use a microscope and oil immersion lens while observing prepared bacterial slides and also to prepare slides for observable bacteria culture from yogart.
Procedure:
Exercise 1: Using the Microscope - Viewing Prepared Slides I started out by reading and reviewed the science lab safety reinforcement Agreement and the instruction to oil immersion lens. After setting up my lab station I view the six prepared slides with 10x and 40x objctives lens. In each slide the differences was magnification and the 40x lans gave a clearer look of the specimen. The observation of slide e was the mirror image when viewed throughthe micoscope. After observations of the six prepared slides. Then I took out oil immersion len. I observed what great amount of magnification and clarity. the slides was highly focused and easy the view individual cells.
Exercise 2: Observing Bacteria Cultures in Yogurt I prepared a teaspoon of yogurt in a cup and placed it inside my closet for 12 to24 hours. Then I viewed the prepared yogurt slide.
Questions:
A. List the following parts of the microscope and describe the function of each:
A: Eye Piece
B: Main Tube
C: Objective Lens
D: Nose piece
E: Stage
F: Removable filter
G: Light Source or Mirror
H: Course Adjustment I: Fine Adjustment Knob
J: Arm
K: Stage knobs
L: Base
B. Define the following microscopy terms:
a. Focus: To move the specimen closer or furthur awy from the objective lens to render a sharper image.
b. Resolution: Is the ability of the lens system to show fine details of the object being observed.
c. Contrast: (Color) Degree of lightness/darkness part of something. Can be flipped depending on the color of the specimen.
C. Describe your observations from fresh yogurt slide you prepared in