• Biology
    Part II: EFFECT OF pH ON ENZYME ACTIVITY (Catechol Oxidase) The active site of an enzyme is affected by the presence of ions that change the charges within the protein molecule. The most favorable pH for an enzyme is called the optimum pH, because it is at this pH that the enzyme is most active. A...
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  • enzyme lab
    throughout the 20 minutes. Therefore, 6 is the optimal pH for catechol oxidase. Lab 6.C Effects of pH on Enzyme Activity   5 Minutes 10 Minutes 15 Minutes 20 Minutes Tube 10 0 0 0 0 Tube 7 2 3 3 3 Tube 6 1 2 3 4 Tube 3 1 2 2 2 This table shows that tube 10 with a pH of 10...
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  • Biology 1010 Enzyme Lab Report
    absorbance amount could then be determined from the effect that different temperatures have on catechol oxidase. Results The results presented are a mean of the class data. The lab manual presented information explaining the effects of catechol oxidase producing benzoquinone. Seen, through the...
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  • Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato
    design and conduct an experiment to demonstrate enzyme activity of Polyphenol Oxidase and Peroxidase when mixed with catechol, caffeic acid, pyrogallad, tyrosine, guacol, and water, to test the effect of inhibitors on these enzymes, to show the specificity of Polyphenol Oxidase and also the effect of...
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  • Enzymes Are Important
    LAB EXERCISE: Enzymes I Catechol Oxidase Laboratory Objectives After completing this lab topic, you should be able to: 1. Define enzyme and describe the activity of enzymes in cells. 2. Differentiate competitive and noncompetitive inhibition. 3. Discuss the effects of varying environmental...
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  • LAB REPORT
    activity of this enzyme was at 40’C because it showed the highest reaction. At very high temperatures enzymes don’t react. They denature in shape and break in structure. Specificity of catechol oxidase for different substrates Hypothesis: The structure of a substrate determines how well an...
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  • The Effect of Substrate Concentration, Enzyme Concentration, Ph and Temperature on Enzyme Activity”
    15. pH solutions Patricia Marcel Biology 1111-17 Lab # 7 Enzymes Results: Table 2B The Effect of Substrate Concentration on Enzymes Activity Tubes dH20 Potato extract Catechol Observations 1 500µl...
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  • Enzymes…the Essential Biocatalysts of Organisms
    Temperature on Catechol Oxidase Activity Tube Distilled Water pH 6 Buffer Potato Extract 1% Catechol Temperature 1 2 mL 1 mL x x 22◦C 2 1 mL 1 mL x 1 mL 22◦C 3 1 mL 1 mL 1 mL x 22◦C 4 x 1 mL 1 mL 1 mL 22◦C 5 x 1 mL 1 mL 1 mL 4◦C 6 x 1 mL 1 mL 1 mL 40◦C 7 x 1 mL 1 mL 1 mL 80◦C Results...
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  • Enzymes Lab
    extract to determine the influence of pH, temperature, and substrate concentration on the rate of enzyme activity. This being said, the increased speed of catechol oxidase should help produce higher concentrations of benzoquinone in the solutions. Methods and Materials The enzyme catechol...
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  • Enzymes
    was done to explain the effect of optimal pH on the enzyme activity and how the activity is affected. The control tubes were from pH 4, pH 6, pH 7, pH 8 and pH10, water and the potato juice were added in the above control/ blank tubes with only a difference of 1 ml of catechol solution added in each...
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  • Bi112 Biology Paper
    The Effect of pH on the Production of Benzoquinone in Solanum tubersum Catalyzed with Catechol Dani Petron Melanie Bilbo Michelle Fox Ivy Victoriano Abstract Both consumers and producers of Solanum tubersum can benefit from determining what pH environment offers the lowest production of...
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  • Formal Lab Report on Enzymes
    shape, they will only work on substrates that fit, and no others. In this lab we utilized Catechol Oxidase as our enzyme, and Catechol as our substrate. When exposed to oxygen catechol gradually changes to benzoquinone, catechol oxidase speeds up this reaction. We tested the effects of temperature...
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  • Factors That Affect Enzyme Reaction Rate
    Lab Report Factors That Affect Enzymes Reaction Rate Name of lab: Effects of temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity Introduction: Enzymes are the most important types of proteins, they act as catalysis (speed up chemical reactions). If...
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  • Temperature Effects on Enzymes
    substrate for the active site).Inhibitors that attach to other parts of the enzyme molecule, perhaps distorting its shape, are said to be non-active site-directed (or non competitive). Most enzymes operate with a very narrow range of temperatures. We tested the activity of Catechol oxidase at 4...
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  • Effect of an Increasing Substrate Concentration on Enzyme Activity Rate
    substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature constant. Our findings...
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  • Inside the cell
    using catechol oxidase as a model to study enzyme function or considering the specific activity of catechol oxidase) 3) State your hypothesis (an educated prediction): Materials and Methods 1) List specific materials used in your experiments (and sources of these materials). Also...
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  • Paper
    Experiment # 7 Enzymes and Cell Function Objectives: The main objective of this laboratory was to understand how an enzyme’s rate of a reaction is affected by different factors. In this laboratory we observed the activity of the enzyme, catechol oxidase, an enzyme in which catalyzes...
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  • Enzyme Kinetics
    competitive inhibitor, thereby impeding the forward reaction. In this experiment, o-diphenol oxidase, an enzyme that causes the browning in fruits, was extracted from banana and reaction rate of this was established with various concentrations of catechol, the substrate, using the Michaelis-Menten...
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  • Enzyme lab
    The Effects of pH, Temperature, Enzyme, and Substrate Concentrations on Benzoquinone Production BIOL 2051 June 10th 2013 Introduction Enzymes are the ultimate catalysts of living things. Enzymes are made of proteins which are structured and directed...
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  • Lab Report About Lab
    : details refer to A.7 Pg 58 of the lab manual (Cail et. al, 2009) Gram stain is based on the physical and biochemical properties of the cell wall. It can be divided into gram positive and negative. Catalase and Oxidase test: refer to A.8 of the lab report (Cail et. al,2009) Catalase test Hydrogen...
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