"Effect Of Temperature On Enzyme Activity Lab Report" Essays and Research Papers

  • Effect Of Temperature On Enzyme Activity Lab Report

    Investigating the Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation, you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed reaction...

    Catalase, Catalysis, Chemical reaction 1031  Words | 4  Pages

  • Effect of temperature on enzyme activity

    Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL, 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start...

    Celsius, Enzyme, Fahrenheit 529  Words | 3  Pages

  • Effect of Temperature on Enzyme Activity

    Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature, the rate of amylase activity is very low. ii) At optimum (=best) temperature, the rate of amylase activity is the...

    Amylase, Amylose, Energy 476  Words | 3  Pages

  • Lab Report on Effect of Enzymes

    Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2), which is a toxic chemical to the human body. When hydrogen peroxide is broken down, the end products are Water (H2O) and Oxygen (O2). In this report, the reaction of catalase to hydrogen peroxide is being tested. Furthermore, the effects of temperature, concentration...

    Acid, Base, Catalase 1054  Words | 5  Pages

  • Effect of Temperature on Enzyme Activity

    Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and...

    Amylase, Catalysis, Energy 1023  Words | 4  Pages

  • Temperature Effects in Enzyme Activity

    Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life's processes would not come so easily. Enzymes are basically proteins, which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape, which makes enzymes different from other proteins. A common enzyme that we have is catalase, which breaks down...

    Catalase, Catalysis, Chemical reaction 809  Words | 3  Pages

  • Lab report on enzyme activity.

    INTRODUCTION Enzymes are a protein serving as a catalyst, a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals, like the different players on a ball team, they have different specific structures and jobs. As one ball player may be very tall and one short, the specific different shape of the active site on an enzyme is unique and prepares it...

    Chemical reaction, Enzyme, Enzymes 1062  Words | 4  Pages

  • Effects of Temperature on Catalase Activity.

    Spring Semester 2013 Lab Report Name: Date: 03/20/2013 Lab: # Purpose of this lab: To test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst, which is to say, it speeds up the reaction. However, extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme is the optimal environment...

    Acid, Base, Catalase 575  Words | 3  Pages

  • Effect of Concentration on Enzyme Activity Lab Report

    Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives, grows, or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of...

    Chemical reaction, Chemistry, Concentration 619  Words | 3  Pages

  • Effect of Temperature, Study and Measure of Enzymes Activity

    Biochemistry CP2085 Effect of Temperature, Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds...

    Chemical potential, Chemical reaction, Chemistry 2441  Words | 7  Pages

  • The effect that temperature has on enzyme activity.

    INVESTIGATING THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase, which is used as a biological catalyst to break down starch, which cannot pass through the gut wall due to the size of the molecules, into smaller ones. Amylase is a carbohydrase, which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific...

    Amylase, Chemical reaction, Enzyme 1615  Words | 6  Pages

  • Enzyme Lab Report

    Biology Lab: Catalase (Enzymes) Abstract In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration...

    Catalase, Catalysis, Chemical equilibrium 1053  Words | 4  Pages

  • The Effect of Concentration, Ph and Temperature on Enzyme Activity

    The Effect of Concentration, pH and Temperature on Enzyme Activity Biology For Majors October 4, 2012 Abstract We examined the reaction an enzyme has when its concentration, pH and temperature are altered. In order to do this, we added different levels of pH into different test tubes with the enzyme (sucrose), and substrate (sucrose), and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature...

    Acid dissociation constant, Buffer solution, Catalysis 2011  Words | 7  Pages

  • Effect of Ph and Temperature on Enzyme Activity in Skeletal Muscle

    Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle, particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions,...

    Adenosine triphosphate, Catalysis, Cellular respiration 1735  Words | 5  Pages

  • The Effect of Temperature on the Enzyme Catalase

    investigate the effect of temperature on the enzyme catalase. The original research question was exploring the effect temperature would have on a yeast catalase reacting with hydrogen peroxide (H2O2). To address the latter question a series of experiments were conducted. The various temperatures experimented with were as follows: 22 degrees Celsius (room temperature), 0 degrees Celsius (freezing), 100 degrees Celsius (boiling), and 37 degrees Celsius. Along with variations in temperature, the experiment...

    Catalysis, Celsius, Chemical reaction 1889  Words | 6  Pages

  • Enzyme Lab Report

     ENZYME LAB REPORT 1. In your own words, define objective qualitative and objective quantitative data. DO NOT USE EXAMPLES AS YOUR SOLE DEFINITION. (3) There are two different types of information that can be obtained from research. The types of information that can be obtained are quantitative and qualitative data. Research results are considered qualitative when the results can be answered with a simple statement of yes or no. Qualitative data does not attempt to give a numerical value; instead...

    Enzyme, Glucose, Hydrolysis 1334  Words | 5  Pages

  • The Effect of Temperature and Ph on Α-Amylase Enzyme Activity

    inside of the amylose coil.The amount of blue complex that starch gives with iodine can be measured by using a spectrophotometer. α-amylases are found in saliva, pancreatic juice, human breast milk, serum and certain tissues such as the liver. This enzyme catalyzes the hydrolysis of α (1-4) linkages in starch by breaking it down to maltose and some glucose. As the starch is broken down, the coiled structure of α-amylase is unfolded. Therefore, iodine will no longer be able to form the blue complex...

    Amylase, Chemical reaction, Enzyme 1379  Words | 5  Pages

  • The Effect of Tempature on Enzyme Activity

     The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process...

    Catalase, Chemical reaction, Chemistry 1649  Words | 8  Pages

  • Enzyme Lab Report

    Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase„³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink „³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration, an increase in reaction temperature, or an increase in buffer pH, then greater...

    Buffer solution, Catalysis, Chemical equilibrium 642  Words | 3  Pages

  • The Effect of Substrate Concentration, Enzyme Concentration, Ph and Temperature on Enzyme Activity”

    Title: “The Effect of Substrate Concentration, Enzyme Concentration, pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea, combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results....

    Acid dissociation constant, Buffer solution, Carbonic acid 1379  Words | 5  Pages

  • College lab report on enzymes

    Lab Ex#8: "Enzymes: Catalysts of Life" INTRODUCTION Enzymes are protein organelles where chemical reactions take place to generate energy within our cells. Without the energy produced from the cell enzyme activity, we would not possess the catalyst activity necessary for energy to produce movement. Each enzyme performs a specific function within our bodies. Those functions performed can be significantly altered with the introduction of variables outside their environment. Variables, such as temperature...

    Catalysis, Chemical equilibrium, Chemical reaction 1294  Words | 6  Pages

  • Effect Of PH On Enzyme Activity

     Effect of pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st, 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our...

    Absolute zero, Anders Celsius, Boiling point 544  Words | 3  Pages

  • enzyme lab report

    Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue, observe the effects of changes in temperature and pH on the activity of an enzyme, perform analyses for the presence of an enzyme in tissues, and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce...

    Base, Catalase, Catalysis 1977  Words | 8  Pages

  • Enzyme Lab

    LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables...

    Acid, Acidosis, Base 1209  Words | 5  Pages

  • Effect of Temperature on the Enzyme Lipase

    LAB 9: DATE: 25TH January, 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost...

    Acid, Catalysis, Chemical reaction 1178  Words | 4  Pages

  • Title : the Affects of Temperature on Enzymes Activity

    Title : the affects of temperature on enzymes activity 1. The Problem: measuring and recording the digestion of carbohydrates by enzyme amylase 2. Independent variable: water bath temperature 3. Dependent variable: the time taken for the carbohydrates to get digested by amylases 4. Controls: the amounts of the carbohydrates and the enzymes in the reaction 5. Quantitative measurement: Time | Temperature | Enzymes concentration | Substrate concentration | Starches Indicators...

    Amylase, Catalysis, Chemical reaction 663  Words | 3  Pages

  • Effect of Temperature on Enzyme Catalase Activity in a Potato

    Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10, 37, 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start...

    Catalase, Energy, Enzyme 1315  Words | 5  Pages

  • Enzyme Lab Report

    experiment was to observe the effects of catalase under varying controlled conditions. The scope of this experiment includes Metabolic processes, such as cellular respiration, and it poisonous byproduct hydrogen peroxide. The methodology includes procedures; multiple variables were tested in specific concentrations; that test the reaction rates of the enzyme catalase over a fixed period of time. The major conclusion was that catalase reacts faster in warm temperatures that are neither freezing nor...

    Catalase, Catalysis, Chemical reaction 1548  Words | 5  Pages

  • Enzymes Lab Report

    Enzymes Lab Report Inroduction In this lab we explore an enzymes activity and how it can be affected by changes to its environment. An enzyme is a protein and is a catalyst to chemical reactions. It helps accelerate reactions by lowering the activation energy, which is needed for reactions in cells to progress at a higher rate. Activation energy is the minimum amount of energy needed for a chemical reaction to occur, yielding products from a given set of reactants. (Unit 7: Enzymes lab) Products...

    Catalysis, Chemical reaction, Chemistry 1657  Words | 5  Pages

  • Investigating the changing effects of temperature on the activity of enzymes

    Investigating the effects of changing temperature on the activity of enzymes Background information: Renin is an enzyme that catalyses the coagulation of milk. It is found in the stomach of many animals and is used in making cheeses and junkets. It is found in the gastric juices or gastric mucosa of many mammals, including humans. In the human stomach, particularly those of infants, rennin works to curdle milk so that pepsin, another stomach enzyme, can further breakdown the proteins into absorbable...

    Chemical reaction, Energy, Enzyme 1503  Words | 6  Pages

  • Effects of Changes in Physical Properties on Enzyme Activity

    Effects of Changes in Physical Properties on Enzyme Activity By Alex Hoffmann First Individual Lab Report Wednesday 7:30-10:15pm 10/24/12 Meghan Duell Abstract The goal of this lab was to determine the effects of certain physical properties on enzyme activity. Enzyme activity was measured by the height of the bubbles that appeared after the enzyme was added which are proportional to the rate of the reaction when time is constant. The fact that enzyme activity is affected by physical...

    Catalysis, Enzyme, Gas 1906  Words | 6  Pages

  • Effects of Enzymes

    The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise, the study of enzyme catalase, we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions.  Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.  Our class performed...

    Chemical reaction, Chemistry, Enzyme 1589  Words | 5  Pages

  • Effect on Enzyme Activity

    EFFECT OF TEMPERATURE,PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature,Ph and substrate concentration on the rate of enzyme activity. Hypothesis ...

    Active site, Catalase, Enzyme 1133  Words | 5  Pages

  • lab report

    Arroyo  Lab report June 11, 2013 Biology 123 Professor K Title: Scientific Investigation of the Peroxidase Enzyme & Temperature Abstract:  In this lab we tested the effect temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the different absorbance levels they produced every 20 seconds for two minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme...

    Catalysis, Chemical reaction, Chemistry 1725  Words | 8  Pages

  • Lab Report Enzyme

    Ching Man 5C(21) Effects of temperature on the activity of lipase Aim: To investigate the effects of temperature on the activity of lipase enzyme on milk which contain fats or lipids. Introduction: Enzyme is a kind of biological catalyst that made up of protein. It can speed up the metabolic reactions on various kinds of substances. Like lipase can break down lipid into glycerol or fatty acids in milk. Since enzyme is made up of protein which easily affected by varies temperature. This experiment...

    Enzyme, Fat, Fatty acid 915  Words | 3  Pages

  • Enzyme Lab Report

    Enzyme lab report. Introduction. An enzyme is a protein molecule that speeds up the rates of chemical reactions by many folds. They recognize, bind, and change specific reactants. They do not change thus can catalyze the same reaction again and again. Activation energy also known as an energy barrier is the amount of energy needed in order to begin a chemical reaction. Catecholase catalyzes the reaction rate of catechol oxidation. Catechol is found beneath the skin of many plants such...

    Activation energy, Catalysis, Chelation therapy 757  Words | 3  Pages

  • Effects of Enzyme

    Effects of Temperature on Enzyme Biology Introduction In order to understand the activity of enzymes at different temperatures the ability of the enzyme to function can be measured. This is important in many applications such as Polymerase Chain Reaction for forensics as well as genetics research where manipulation of temperature-dependent enzymes allows for replication of DNA segments. Bennett states, “when the energy - measured...

    Absolute zero, Celsius, Chemical reaction 776  Words | 3  Pages

  • Enzyme Activity

    LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity Pam Campbell Id 0002337 Dr. Murphy Nmezi August 9, 2011 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable...

    Acidosis, Base, Blood 1132  Words | 4  Pages

  • Enzyme lab report

    Name : Andi Nadya Amanda Period : 4 Grade : 11 Enzyme Lab Report Question How heats effect the length of reaction time of an enzyme? Hypothesis I think the heat will make the length of reaction time of an enzyme become slowly. Heat is one of a way to denature the substrate. It means the heat will break down the structure of substrate in order the reaction of enzymes that we activated into it become slowly. Method for Collecting Data First I will record the length of reaction...

    Boiling, Enzyme, Heat 807  Words | 5  Pages

  • lab report enzyme

    Ching Man 5C(21) Effects of temperature on the activity of lipase Aim: To investigate the effects of temperature on the activity of lipase enzyme on milk which contain fats or lipids. Introduction: Enzyme is a kind of biological catalyst that made up of protein. It can speed up the metabolic reactions on various kinds of substances. Like lipase can break down lipid into glycerol or fatty acids in milk. Since enzyme is made up of protein which easily affected by varies temperature. This experiment...

    Enzyme, Fat, Fatty acid 915  Words | 3  Pages

  • Biology Enzyme Lab

    Enzyme Lab 6 03/13/2013 Report by Mary Jo Anthony I. Introduction II. Materials and Methods III. Results IV. Conclusion and Discussion Introduction Background Information: This lab allowed us to study chemical reactions and how catalysts will affect the rate of these reactions. The reaction we studied is the breakdown of hydrogen peroxide to water and oxygen and it is vital to life. The molecule hydrogen peroxide is a molecule that is toxic...

    Acid, Base, Catalase 2441  Words | 7  Pages

  • enzyme lab

    Lab 6.C Hypothesis: If enzyme activity is affected by the pH of a solution, then the enzymes will experience the greatest activity at a pH of 6. Variables: Independent Variables Dependent Variable Controls Four different pH values (10, 7, 6, and 3) Change in color of the solution The amount of potato extract, pH solution, and catechol used (1 cm +/- .1cm) Size of the test tubes Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with...

    Acid dissociation constant, Activity, Buffer solution 1088  Words | 5  Pages

  • Enzyme Lab

    Abstract Enzyme catalysis was observed in order to analyze how changes in temperature, pH, enzyme concentration, and substrate concentration affected an enzyme-catalyzed reaction. This experiment analyzed the rate of enzyme-catalyzed reactions and observed the correlation between catalase activity and products formed. It was found out that the rate of an enzyme-catalyzed reaction starts off rapidly, decreases, and levels off or completely stops, and can be further affected by environmental factors...

    Catalysis, Cell, Digestion 1135  Words | 4  Pages

  • An Investigation Into the Effects of Temperature on Enzyme Action

    An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems, for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate...

    Boiling point, Celsius, Enzyme 1249  Words | 3  Pages

  • Enzyme Lab Report

    Enzyme Reaction to Variable Elizabeth Alvidrez University of Texas at El Paso Abstract: An enzyme is a protein that serves as a biological catalyst (Denniston, 2007). Metabolic reactions happen with in cells. Enzymes are proteins that are used to speed up these reactions without being consumed by them (Mader, 2010). Catalase is a catalyst that digests potent hydrogen peroxide and converts it into H2O and O (Campbell Reese, 2008). The environment plays an important role...

    Buffer solution, Celsius, Chemical reaction 1403  Words | 5  Pages

  • lab report on enzymes

    Enzymes What Are Enzymes? Substances that speed up chemical reactions are called catalysts. Organic catalysts are called enzymes. Enzymes are specific for one particular reaction or group of related reactions. Many reactions cannot occur without the correct enzyme present. They are often named by adding "ase" to the name of the substrate. Example: Dehydrogenases are enzymes that remove hydrogen. Induced-fit Theory The shape of the enzyme must match the shape of the substrate...

    Active site, Catalysis, Chemical reaction 570  Words | 3  Pages

  • Lab 6 enzymes

    Lab 6: Enzymes . header Purpose: The purpose of this lab is to test for enzyme activity, look at enzyme specificity, and how temperature affects enzyme activity. Time need to perform this lab: approximately 3 hours Preparation FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment, write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a...

    Boiling, Catalase, Drinkware 1000  Words | 5  Pages

  • The effect of enzyme concentration, substrate concentration, pH, and temperature on the enzyme catalase.

    The effect of enzyme concentration, substrate concentration, pH, and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes, being highly specific, only fit with one certain substrate. Enzymes and substrates...

    Catalase, Catalysis, Chemical equilibrium 864  Words | 3  Pages

  • Formal Lab Report on Enzymes

    Lab #5-Enzymes NAME DATE LAB PERIOD Introduction Enzymes are proteins, though highly complex and diverse, they serve one basic function; to work as an organic catalyst. A catalyst, as defined by Merriam-Webster dictionary, is a substance that enables a chemical reaction to proceed at a usually faster rate ("Catalyst-Definition and more."). They function by reducing the activation energy, or energy required to start a reaction. The way enzymatic reaction works cannot be altered, but the...

    Catalysis, Chemical reaction, Enzyme 1105  Words | 4  Pages

  • Lab Report Enzymes

    test the enzyme activity of Catecholase the enzyme in potato juice, in many different conditions such as varying temperatures, ranging pH, and concentration of the enzyme, as well as concentration of the substrate. Most reactions are very slow; enzymes are used to speed up reactions. Enzymes have an active site that a substrate attaches to, but changes temperature, pH, and concentration can alter the active site (Helms, 1998). Organisms have a specific range of temperature in which enzymes work. Enzymes...

    Acid dissociation constant, Activity, Buffer solution 922  Words | 5  Pages

  • Factors affecting the rate of Enzyme activity

    Shafagh Ranjbar Dr. Danielle Lab Report (2) 18.Sep.2013 Factors Affecting the Rate of Enzyme Activity The Purpose of doing this experiment is “how factors affect on the rate of enzyme activity.” “We examine the affects of enzyme activity when exposing enzymes to different substrates, PH and temperature. In this experiment a type of enzyme called Peroxides has been used. Peroxidase is the enzymes are found in living organism so, it can be founded in both liver and potato...

    Catalase, Enzyme, Glucose 1642  Words | 5  Pages

  • Enzyme Lab

    Sample Lab Report: Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include:  background information about concepts involved in the lab,  statement of purpose of the lab  identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in...

    Catalase, Chemical element, Enzyme 716  Words | 3  Pages

  • Effect of Concentration on Enzyme Activity Lab Report

    Explain the technique by which copper can be purified. What is a use of pure copper? 19) Explain the processes of phytomining and bioleaching. 20) Why is recycling beneficial to the environment? 21) What % of iron comes from the blast furnace? What effect does this have on its properties? 22) Give some properties of low-carbon, high-carbon and stainless steels. 23) Why might an alloy of aluminium be preferable to the pure metal in aeroplane manufacture? 24) What name is given to the metals in the...

    Atom, Carbon, Carbon dioxide 799  Words | 3  Pages

  • Enzymes Laboratory Report

    ENZYMES LABORATORY REPORT Introduction The utilization of any complex molecule for energy by an organism is dependent on a process called hydrolysis. Hydrolysis breaks complex molecules into simpler molecules using water. Similarly, the process that is the reverse of this is called dehydration synthesis, which removes water from simpler molecules. However, because hydrolysis occurs very slowly, living organisms use biochemical’s called enzymes to speed up the reaction. In this lab exercise...

    Chemistry, Enzyme, Gas 1255  Words | 4  Pages

  • Effects of Temperature, Ph, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity

    Effects of Temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes, proteins that act as catalysts, are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes, the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol...

    Base, Buffer solution, Catalysis 2348  Words | 7  Pages

  • Biology 1010 Enzyme Lab Report

    are produced by the oxidation of phenolic compound of catechol. Enzymes are used to speed up chemical reactions in cells (Danyk, H, 2013). The enzyme catechol oxidase is used to speed up the production of benzoquinone which is to help in infection prevention. In this study catechol oxidase was combined with potato juice extract and water. To provide more results of the enzymes productivity patterns the solutions; temperatures were changed, alterations were made in pH, and the amount of concentration...

    Acid, Enzyme, Enzymes 1226  Words | 4  Pages

  • Enzyme Lab Report

    MISEP Chemistry 512 – Jacobs Enzyme Catalyst Lab - Formal Report – August 8, 2007 ABSTRACT This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased, then the reaction rate would also increase. To test our question, we varied a combination of substrate and buffer, totaling 6mL, with a constant amount of 2 drops of catalyst. The enzyme catalyst, peroxidase, increased...

    Activation energy, Catalysis, Chemical equilibrium 1107  Words | 4  Pages

  • Effect of temperature on Catalase activity

    THE EFFECT OF TEMPERATURE ON CATALASE RESEARCH QUESTION: What is the effect of various temperatures, 0°C, room temperature, 37°C, 50°C, 60°C, on the number of oxygen gas bubbles liberated, in a decomposition reaction between the enzyme Catalase, obtained from crushed mung beans, and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins...

    Catalase, Chemical reaction, Enzyme 849  Words | 5  Pages

  • Bio Lab Report 1—— Enzyme

    Ye Tao BISC220-13155 The Effect of Temperature on the Digestion of Starch by Activity of Enzyme α-Amylase: Observation of Rate of Starch Disappearance through Iodine Test Introduction An enzyme is a type of protein that, through its own structure including hydrogen bonds, acts like a biological catalyst and is able to accelerate the biochemical reaction rate by lowering the activation energy of the whole process, without which cells could hardly practice any physiological functions within human...

    Amylase, Catalysis, Chemical kinetics 1822  Words | 5  Pages

  • Factors Influencing Enzyme Activity

    exercise we studied enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Each lab was assigned...

    Catalase, Catalysis, Chemical reaction 1808  Words | 5  Pages

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