LABORATORY REPORT Activity 11: Homeostatic Imbalances of Thyroid Function Name: Instructor: Date: PREDICTIONS 1. TSH levels in patients with primary hypothyroidism are: 2. Thyroxine (T4) levels in patients with primary hypothyroidism are: 3. TSH levels in patients with secondary hypothyroidism are: 4. Thyroxine (T4) levels in patients with secondary hypothyroidism are: 5. TSH levels in patients with hyperthyroidism are: 6. Thyroxine (T4) levels in patients with hyperthyroidism are:
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How Temperature Affects Amylase Activity Abstract This study of the effect of temperature on the enzyme amylase was performed to determine the relationship between the enzyme amylase and temperature. The rate of reaction was found to increase as the temperature of the environment was raised. As the temperature was raised from 5°C‚ 20°C‚ 35°C and finally to 80°C the rate of reaction followed this trend and also increased. However as predicted in the hypothesis of this experiment when the temperature
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The Determination of the Effect of pH on Amylase Activity Grace Chung Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes‚ including amylase‚ function best at a certain optimal pH. Therefore‚ in this experiment‚ the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to
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Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
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Effects of Temperature and pH on Catalase Activity INTRODUCTION Enzymes are organic catalysts that spur metabolic reactions. The presence of an enzyme within a cell is essential in order for any sort of reaction to take place. All enzymes are complex proteins that act in an organism’s closely controlled internal environment. In such a homeostatic environment‚ the temperature and the pH (concentration of hydrogen ions)‚ remain within a fairly narrow range. Extreme variations in pH and temperature
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experiment was to determine the effect of temperature on the rate of movement of Porcellio scaber. Our prediction was that P. scaber would have faster rates of movement in hotter temperatures. We expected this response as P. scaber is an ectotherm. Hence‚ its body temperature is directly determined by the temperature of its environment (Mellanby 1939). Løkke and van Gestel (1998) stated that the rate of the metabolism is controlled by the environmental temperature for ectotherms. As seen by Wieser
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Locomotor activity The Locomotor activity (Pákáski and Kálmán‚ 2008) refers to the movement of spontaneous locomotor and exploratory activity in rodents. LMA is a simple performed measurement of behavior to detect the effects of drugs‚ genetic manipulation‚ etc.‚ on motor function by measuring horizontal‚ vertical and stereotyped behavior in rodents. Several measures of locomotor activity in rats‚ including the distance covered‚ movement time and speed of progression. This test is sensitive to
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To begin the analysis‚ the properties of the air at the base of the tower were examined. The wet and dry bulb temperatures of the air at the inlet were used to find the humidity‚ humid volume‚ and enthalpy of dry air. For example‚ in the first trial the dry bulb temperature was 22.5⁰C and the wet bulb was 16.5⁰C‚ and the properties found from the psychrometric chart are listed below. H_bottom=0.0095 kg/(kg dry air) V_bottom=0.85 m^3/(kg dry air) h_bottom=47 kg/(kg dry air) Then‚ the cross-sectional
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Amylopectin The absorbance value(x) read from cuvette containing starch and water represents the total amount of starch-iodine complex. The absorbance value(y) read from cuvette containing starch‚ water and α-amylase at the respective temperature or pH represents the amount of starch-iodine complex which is left after the enzyme has hydrolyzed the starch.In order to get the amount of product;P (maltose and glucose) formed‚ need to subtract the (y) value from the (x) value.
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In this lab‚ I was testing different temperatures and how it affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than
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