Catechol Oxidase Lab Report
Studying the Rate of Reaction of catechol oxidase and how it affects pH levels
Introduction:
In this lab, we studied the activity of an enzyme that is found in fruits and vegetables called catecholase or catechol oxidase. An enzyme is a protein molecule that is a catalyst. Catechol oxidase is the enzyme in fruits and vegetables that turns them that undesirable brownish color; also commonly referred to as bruising or bruised. When walking through your regular grocery store and you find yourself in the produce section, what is it that you look for before putting that produce in the bag for weighing? The size, the smell, the color? Most of us would have to say that if they grabbed an apple that had a bruised body, they would return that apple to the pile in search for that perfect skinned apple. This is why studying catechol oxidase is so important!
Let’s take a look at population numbers and why …show more content…
Well as it would have it, all enzymes are affected by changes in pH. Not only can it change the shape of the structure of the enzyme, but it can also change the shape or charges of the substrate as well. That can make it so the substrate is unable to bind to the active site making it unable to go through catalysis. So they work in what’s called the “lock and key” manner with the substrate. When the optimum pH level of an enzyme is met, it allows it to lock with the substrate to form the catalyst.
The optimum pH is where the enzyme is the most active. But just because one enzyme is most active at a certain point, doesn’t mean that other enzymes are the same. In fact, the optimum pH can differ greatly from enzyme to enzyme. Please look to the left for what optimum pH will look like on a graph (5). As you can see, at the highest point of the bell curve is where the optimum pH of that enzyme is. The optimal pH allows the reaction between the enzyme and substrate to continue
Introduction:
In this lab, we studied the activity of an enzyme that is found in fruits and vegetables called catecholase or catechol oxidase. An enzyme is a protein molecule that is a catalyst. Catechol oxidase is the enzyme in fruits and vegetables that turns them that undesirable brownish color; also commonly referred to as bruising or bruised. When walking through your regular grocery store and you find yourself in the produce section, what is it that you look for before putting that produce in the bag for weighing? The size, the smell, the color? Most of us would have to say that if they grabbed an apple that had a bruised body, they would return that apple to the pile in search for that perfect skinned apple. This is why studying catechol oxidase is so important!
Let’s take a look at population numbers and why …show more content…
Well as it would have it, all enzymes are affected by changes in pH. Not only can it change the shape of the structure of the enzyme, but it can also change the shape or charges of the substrate as well. That can make it so the substrate is unable to bind to the active site making it unable to go through catalysis. So they work in what’s called the “lock and key” manner with the substrate. When the optimum pH level of an enzyme is met, it allows it to lock with the substrate to form the catalyst.
The optimum pH is where the enzyme is the most active. But just because one enzyme is most active at a certain point, doesn’t mean that other enzymes are the same. In fact, the optimum pH can differ greatly from enzyme to enzyme. Please look to the left for what optimum pH will look like on a graph (5). As you can see, at the highest point of the bell curve is where the optimum pH of that enzyme is. The optimal pH allows the reaction between the enzyme and substrate to continue