CHEMISTRY 221 LABORATORY�QUANTITATIVE ANALYSIS EXPERIMENT: DETERMINATION OF ASCORBIC ACID IN VITAMIN C TABLETS THEORY: Ascorbic acid‚ C6H8O6‚ MM = 176.13‚ can be oxidized to form dehydroascorbic acid by bromine via the following reaction: The bromine or iodine used in the titration is generated by adding an excess amount of KBr or KI to an acidified solution of the sample according to the following reaction: BrO3- + 5 Br- + 6 H+ 3 Br2 + 3 H2O IO3- + 5 I- + 6 H+
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: THE VITAMIN C CONTENT OF FRUIT JUICES AND TABLETS DATE OF EXPERIMENT : 1ST AUGUST 2013 DATE OF SUBMISSION : 27TH AUGUST 2013 LECTURER : PUAN ZAKIAH BT. ZAKARIA OBJECTIVE * To determine the vitamin C content in fresh fruit juice * To determine the vitamin C content in commercial fruit juice * To determine the vitamin C content in tablet
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person needs a daily intake of Vitamin C to stay healthy; therefore‚ one should be aware of what fruits he/she should ingest daily or more days to obtain stronger stamina and the abundance of antioxidants in the body to reverse oxidation. Vitamin C or L-ascorbic acid is essential for normal growth of humans and it helps in the repair and reconstruction of body tissues lost over time. It is an antioxidant that blocks the damage caused by free radicals. People who take Vitamin C daily tend to build up a
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IB Biology Lab – Vitamin C content of food Aim: To compare the vitamin C content of different fruits using the decolourisation of DCPIP from blue to clear as the indicator. • You first need to estimate the volume of a known concentration of vitamin C solution required to decolourise 2 cm3 of DCPIP solution. • Then you need to determine the volume of fruit juice that decolourises 2 cm3 of DCPIP solution. This gives an estimate of the concentration of vitamin C in fruit juice. • This
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the vitamin C content of different fruit juices. To identify which fruit juices has higher vitamin C content and the lowest vitamin C content. To determine the standard curve of vitamin C based on percentage of vitamin C. To differentiate between fresh juices and carton juices. To consider the reliability and validity of the results. PROBLEM STATEMENT Which type of fruit juices has the most vitamin C content? HYPOTHESIS The smaller the amount of the fruit juices to decolourise DCPIP solution
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Investigating Vitamin C Background Vitamin C is found in green vegetables‚ fruits‚ and potatoes. It is essential for a healthy diet. The chemical name for vitamin C is ascorbic acid. Ascorbic acid is a good reducing agent and therefore it is easily oxidised. Methods for the detection of vitamin C involve titrating it against a solution of an oxidising agent. Where to start There are several oxidising agents that can be used and a commonly used one is 2‚6-dichlorophenol-indophenol or DCPIP. You need
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Estimating the Vitamin C Concentration in Fruit Juices: A Redox Titration Prelab Assignment – Please turn your answers in on GaView prior to lab 1. How will you prepare a .02% (m/v) NBS solution? Calculations should be in your notebook before arriving to lab. 2. Provide the structure of vitamin C in its reduced form (ascorbic acid)‚ its ionized form (ascorbate ion- this is at equilibrium with reduced form when dissolved)‚ and its oxidized form (dehydroascorbate). 3. Why do you
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Investigating the vitamin C content of fruits and fruit juice Aim: In this investigation‚ I aim to measure and compare whether there is a higher content of vitamin C in a fruit or a juice by measuring the volume of the sample required to decolourise a solution of dichlorophenolindophenol (DCPIP). Introduction: For this experiment‚ I aim to investigate and compare the content of vitamin C in the fruit against the fruit juice. The theory of this method is a titration with dichlorophenolindophenol (DCPIP). DCPIP
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Practical Task: To compare the concentration of vitamin C in different brands of orange juice. Juice sample provided: 0.1% vitamin C solution‚ fresh orange juice‚ You C 1000 orange water. Aim of investigation: To compare and verify the amount of vitamin C in Mr. Juicy and You C 1000 orange juice. Introduction: Problem: To compare the concentration of vitamin C Hypothesis: Concentration of vitamin C in Mr. Juicy is about 0.02 % Concentration of vitamin C in You C 1000 orange water is about
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Abstract: The purpose of this experiment was to determine the concentration of an unknown amount of copper sulfate using the spectronic 20. Four solutions of different concentrations of H₂O and CuSO₄ were put into the spectronic 20 to observe their absorption of light. Then‚ a graph was created to plot down the measurements. Using the calibration curve of the graph‚ the concentration of the unknown substance was found out to be .12M. Background/Introduction: Beer’s law (which states that
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