the SDS-PAGE and Simple-PAGE gels were prepared with 3.25mL water‚ 2.0mL 4X resolving buffer‚ 2.7mL 40% acrylamide solution‚ 80μL 10% APS‚ and 3μL TEMED. The stacking layers for the SDS-PAGE and Simple-PAGE gels were prepared with 4.6mL water‚ 2.0mL 4X stacking buffer‚ 1.3mL 40% acrylamide solution‚ 48μL 10% APS‚ and 5μL TEMED. The SDS-PAGE gel used the buffers containing SDS and the Simple-PAGE gels used the buffers without SDS. The gels were run at 100 V for about 60
Premium Metabolism Protein DNA
during the lab session Procedure Part A This lab was performed by the group members as they closed both low and high flow rotameters (Fig-2) and filled the tank with water. Then‚ the large rotameter was opened when the water in the tank had reached the internal baffle‚ so the flow from the tank was reduced by doing so. One member was in charge of adjusting the level of the water so it was always maintained over the baffle’s level during the lab (Fig-1). The first part of the lab was done
Premium
Lab Report Diffusion is - One of two kinds of passive transport‚ - Diffusion can transport ions from higher concentration to lower concentration region without any other forces. - A net movement of molecules in and out of cell membrane - Diffusion can be affected by the steepness of the concentration gradient. Lab question: Is the rate of diffusion influenced by the presence of second molecule? Prediction: the rate of diffusion is influenced by the presence of second molecule
Premium Diffusion Molecular diffusion Chemistry
between the amount of substrate in the assay solution and the rate of the reaction when the enzyme and buffer in the assay are held constant were experimented. We analyzed the change in absorbencies over time for varying substrate concentrations. There were four experimental assays which contained 1% enzyme solution‚ substrate solution of 0%‚ 1%‚ 2%‚ and 3% concentrations‚ guaiacol‚ and pH 7 buffer. At 2% concentration there was a greater enzymatic activity and at 3% concentration enzymatic activity
Premium Enzyme Chemical reaction Chemistry
December 8‚ 2013 Soil Analysis Lab Report Intro: Soil is produced over hundreds of years through the weathering of rocks and the decomposition of organic material. Soil contains nutrients that are taken from decomposed animals and plants which turn to detritus. Nutrients such as nitrogen are added to the soil in these ways by organisms such as nitrogen fixing bacteria. Soil is important because it is the fuel to the plants that we need in order to survive and for all species to survive. Soil
Premium Soil Nitrogen Plant
Anxiety Lab Report Abstract: The aim of this study is to investigate the relationship between cognitive anxiety‚ somatic anxiety and self confidence in two different situations; non-stressful and stressful. It is to examine the effects on anxiety of a simple golf putting task. The stressor used in this experiment can be described as situational‚ namely the stressor of social comparison‚ the type of stressor that leads many performers to question their own ability which in turn evokes symptoms
Premium Psychology Anxiety Generalized anxiety disorder
BIOLOGY LAB REPORT (UNIT 7: ENZYMES) GENERAL Enzymes are protein that acts as catalyst‚ lowering the activation energy need for reactions to progress in cells. The reaction can still occur without the presence of the enzyme‚ but at a much slower rate. The activation energy is the minimum amount of energy need for a chemical reaction to occur‚ yielding from a given set of reactants. In enzymatic reactions‚ we have substrates which are reactants of reaction bound to an enzyme. While an active site
Premium Enzyme Catalysis
Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
Premium Leaf Color Chlorophyll
------------------------------------------------- Module title: EUC_4_005 Design and Practice ------------------------------------------------- Occurrence Number: [Overtype your occurrence Number here] ------------------------------------------------- Lab Group Number: [Overtype your lab group number here] ------------------------------------------------- Course: [Overtype name of course here] ------------------------------------------------- ------------------------------------------------- Student Name: [Overtype
Premium Typography Present tense Citation
Antibiotic Lab Report By: Sierra Toll Hour: 6 Title: The effects of antibiotics on the growth of Escherichia Coli Problem: How do antibiotics affect the growth of E coli? Hypothesis: If Penicillin is applied to the bacteria‚ then the zone of inhibition will be larger. Background: The purpose of this lab was to see how effective a certain antibiotic is to kill E coli. The lab tested three antibiotics‚ Penicillin‚ Erythromycin‚ and Neomycin. The zone of inhibition‚ which is the amount of space
Free Bacteria Antibiotic resistance