Macromolecules Lab Report
Background: Lipid and protein composition varies in tissue types.
Results: When comparing liver, muscle, and adipose tissue, adipose tissue had the highest percent lipid and liver tissue had the highest percent protein.
Conclusion: Based on analysis of the gels, the liver appeared to have the most variety in proteins when comparing liver, muscle, and adipose tissue.
Significance: In order to determine the composition of a tissue, specific macromolecules can be extracted, quantified, and analyzed.
ABSTRACT Tissue types have a variety of compositions, and one way to describe these differences is by comparing macromolecule content. The four macromolecules – lipids, proteins, carbohydrates, and nucleic acids – can be quantified and analyzed. …show more content…
Lipids are macromolecules made up of fatty acids connected by ester bonds. Proteins, the most abundant and versatile macromolecule, is made up of a chain of amino acids connected by peptide bonds. Carbohydrates are sugars made up of monosaccharides connected by glycosidic bonds. Nucleic acids are made up of nucleotides, and examples include DNA and RNA. Each tissue in the body has a different amounts of the four macromolecules. Based on a study done by Baker, the percentage of lipid in adipose tissue in humans at birth is about 40% and increases up to about 75% with age (1). According to Pawan and Clode, The percentage of protein in adipose tissue in humans is about 2.1% (6).
The Folch Method can be used to extract lipids from tissue samples. Lipids are hydrophobic and thus do not dissolve in polar substances such as water but can dissolve in organic solvents. The Lowry Method is a commonly used and highly reproducible method of protein …show more content…
Polyacrylamide gel electrophoresis (PAGE) is a commonly used method for separating proteins. Both SDS-PAGE and Simple-PAGE gels were prepared. The resolving layers for the SDS-PAGE and Simple-PAGE gels were prepared with 3.25mL water, 2.0mL 4X resolving buffer, 2.7mL 40% acrylamide solution, 80μL 10% APS, and 3μL TEMED. The stacking layers for the SDS-PAGE and Simple-PAGE gels were prepared with 4.6mL water, 2.0mL 4X stacking buffer, 1.3mL 40% acrylamide solution, 48μL 10% APS, and 5μL TEMED. The SDS-PAGE gel used the buffers containing SDS and the Simple-PAGE gels used the buffers without SDS. The gels were run at 100 V for about 60
Results: When comparing liver, muscle, and adipose tissue, adipose tissue had the highest percent lipid and liver tissue had the highest percent protein.
Conclusion: Based on analysis of the gels, the liver appeared to have the most variety in proteins when comparing liver, muscle, and adipose tissue.
Significance: In order to determine the composition of a tissue, specific macromolecules can be extracted, quantified, and analyzed.
ABSTRACT Tissue types have a variety of compositions, and one way to describe these differences is by comparing macromolecule content. The four macromolecules – lipids, proteins, carbohydrates, and nucleic acids – can be quantified and analyzed. …show more content…
Lipids are macromolecules made up of fatty acids connected by ester bonds. Proteins, the most abundant and versatile macromolecule, is made up of a chain of amino acids connected by peptide bonds. Carbohydrates are sugars made up of monosaccharides connected by glycosidic bonds. Nucleic acids are made up of nucleotides, and examples include DNA and RNA. Each tissue in the body has a different amounts of the four macromolecules. Based on a study done by Baker, the percentage of lipid in adipose tissue in humans at birth is about 40% and increases up to about 75% with age (1). According to Pawan and Clode, The percentage of protein in adipose tissue in humans is about 2.1% (6).
The Folch Method can be used to extract lipids from tissue samples. Lipids are hydrophobic and thus do not dissolve in polar substances such as water but can dissolve in organic solvents. The Lowry Method is a commonly used and highly reproducible method of protein …show more content…
Polyacrylamide gel electrophoresis (PAGE) is a commonly used method for separating proteins. Both SDS-PAGE and Simple-PAGE gels were prepared. The resolving layers for the SDS-PAGE and Simple-PAGE gels were prepared with 3.25mL water, 2.0mL 4X resolving buffer, 2.7mL 40% acrylamide solution, 80μL 10% APS, and 3μL TEMED. The stacking layers for the SDS-PAGE and Simple-PAGE gels were prepared with 4.6mL water, 2.0mL 4X stacking buffer, 1.3mL 40% acrylamide solution, 48μL 10% APS, and 5μL TEMED. The SDS-PAGE gel used the buffers containing SDS and the Simple-PAGE gels used the buffers without SDS. The gels were run at 100 V for about 60