Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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Exploring the diversity‚ abundance‚ and variability of diatom-associated bacteria in the oligotrophic ocean I. Abstract The ecology of diatoms may be better explained by conceptualizing them as composite organisms consisting of the host cell and its bacterial associates. Our previous investigated diatom-bacterial interactions at the single-cell level found that bacterial assemblages varied substantially even among closely related individual host cells. The bacterial assemblages associated with single
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microorganisms will grow at the top of the tube‚ but will also be dispersed throughout as well. B. Discuss the temperature requirements of the organisms (you grew) in this lab. Both Staphylococcus epidermis and Lactobacillus acidophilus are mesophiles. Mesophiles grow best between 25- 45 degrees Celsius (Alonzo‚ n.d.). In this experiment both bacteria have grown easily at a maintained room temperature of 26 Celsius. C. Discuss under what conditions you would want to control the growth of microbes.
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are chemicals or natural substances such as bacteria‚ fungi‚ as well as synthetic drugs; that inhibit or kill microbes in small amounts. Chemotherapeutic agents are antimicrobial chemicals (natural or synthetic) that can be used internally or may be absorbed. The purpose of antimicrobial drugs is either to inhibit virus replication‚ without harming host tissues or to interrupt the cell processes or structures of bacteria‚ fungi‚ and protozoa. This lab has brought great interest to me because this
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Lab report As part of my module Nip1002 I was required to perform a set of observational skills which included; pulse‚ blood pressure‚ respirations‚ hand washing and urinalysis and then compare them to previous results. In this lab report I am going to discuss blood pressure in detail. Procedure * Explain the procedure to the patient and gain consent * Wash hands thoroughly following the hand wash technique to prevent the transfer of bacteria * Clean equipment e.g. blood pressure cuff
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SAMPLE DESCRIPTIVE LAB REPORT Examination of Protozoan Cultures to Determine Cellular Structure and Motion Pattern Abstract Protozoans are unicellular eukaryotes with either plant- or animal-like characteristics. Through careful observation‚ we analyzed various protozoan cultures in order to identify characteristics associated with cell structure and movement of these one-celled organisms. We found that Protists exhibit certain characteristics that allow them to be categorized into different groups
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Lab Report Microbiology Introduction Bacteria can be found almost anywhere. For human life‚ some help us‚ some hurt us‚ and others are neutral. It is now known that good bacteria‚ or normal microflora‚ can reach 1014 microbial cells. This is far more than the 1013 cells that make up the human body (Tannock‚ 1995). The total number of bacteria on Earth is estimated to be around 4-6 x 1030 (Horner-Devine‚ 2004). It is important to know the extent of bacteria‚ how they live‚ and how they are
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This lab report will be detailing the steps taken and the results discovered when using spectrophotometry to determine the percentage of copper in a copper-clad penny and the thicknes of the copper layer on the copper-clad penny. After 1982‚ copper coating has been used in the creation of the penny because the cost of pure copper has increase to the point that the amount needed t omake a penny cost far more than the actual value of the penny. This lab allowed us to see just how much copper coating
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Institute Physics Course 2014 - Rocket Lab Report Title: RockSim and Electronic Altimeter Measurement Techniques Name: Scarlet Henriquez Email: scarletpatricia94@aol.com LAB SESSION ___8____Assignment _____7_____ Due Date: ___7/8/14______ Objective: Launch a smaller rocket to learn how to compare RockSim predictions (computer based) to an actual instrument measurement carried by the rocket (Electronic Altimeter). This Lab shows how better the electronic measurement
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