Introduction This lab is purposed to familiarize basic equipment and techniques used in the study of microorganisms. In addition‚ learn some basic techniques used in identifying prokaryotes and make and view microscope slides of some common prokaryotes. In this lab ‚ I worked two experiments ‚ the first one is cultivation bacteria "colony "‚ and When microorganisms are cultivated in the laboratory‚ a growth environment
Premium Bacteria Microbiology Microorganism
Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
Premium Gram staining Bacteria Staining
Bacteria Lab Write-Up Jianna Liu P8 Purpose: The purpose of this lab is to find out which substance‚ alcohol‚ antibacterial soap‚ water‚ or hydrogen peroxide‚ is the best at preventing the growth of bacteria. Hypothesis: Antibacterial soap will be the best because soap is a substance that people use to wash their hands because it takes away the germs. Even the name‚ antibacterial soap‚ suggests that it fights off bacteria. Hydrogen peroxide is second because it is used
Premium Oxygen Bacteria Agar plate
Lab: The Bacteria Around You James Brunet Ms Owen October 14th‚ 2012 Part 1 Purpose To culture and observe the various types of bacteria found around Canterbury High School. Materials and Methods Refer to pages 422-425 of Biology 11 McGraw-Hill Ryerson and the handout “Gram Staining Procedure”. Observations Table 1: Locations of Bacteria Samples Quadrant | Location of Sample Obtained | 1 | Floor | 2 | Water fountain head | 3 | Auditorium Chair | 4 | Inside of Boys’ Bathroom
Premium Bacteria
------------------------------------------------- Isolation and Culture of Zoosporic Fungi using Baiting Technique ------------------------------------------------- Rillera DP ‚ Talibsao K ‚ Talucod AC and Tan P Department of Biological Sciences‚ College of Science‚ University of Santo Tomas‚ España Street‚ Manila 108 ------------------------------------------------- Zoosporic fungi belonging to class Oomycetes of Kingdom Chromista and Phylum Chytridiomycota of Kingdom Eumycota‚ were
Premium Fungus Slime mold
BSE-Biological Sciences III-3 Microbiology (Lecture) Microbiology Written Report REPORT NO. 1 Prokaryotes: Bacteria Most of us have been conditioned to think of bacteria as invisible‚ potentially harmful little creatures. Actually‚ relatively few species of bacteria cause disease in humans‚ animals‚ plants‚ or any other organisms. In fact‚ all organisms made up of eukaryotic cells probably evolved from bacteria-like organisms‚ which were some of the earliest forms of life. In this chapter
Premium Bacteria
{draw:frame} Aseptic technique was developed by Joseph Lister in 1867. He used diluted phenol to cleanse surgical wounds and equipment. He also used carbolic acid aerosol to prevent harmful microorganisms from entering the surgical field or contaminating the patient. Aseptic technique includes hand washing‚ the use of sterile gloves‚ mask‚ and gowns‚ sterilization of surgical instruments and other equipment‚ and the use of disinfectants‚ including antiseptics. The definition of asepsis is: 1)
Premium
Laboratory Techniques & Measurements Observations from Procedures Data Table 1 – Length measurements Object|Length (cm)|Length (mm)| CD|12|120| Key|5|50| Spoon|17|170| Fork|19|190| Metric Ruler|30|300| Data Table 2 – Temperature measurements Hot water from tap (44ºC )|Boiling water (70ºC)|Boiling water – 5 minutes (100ºC)| ]111.2 F|158 F|212 F| Cold water from tap (24ºC)|Ice water (5ºC)|Ice water – 5 minutes (0ºC)| 75.2 F|41 F|32 F| Data Table 3 – Mass measurements
Premium Water Temperature Fahrenheit
Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques used were
Premium Escherichia coli Staining Gram staining
Treatment of Results 1) What is the role of 0.25M sucrose as the medium for the fractionation process? Cold sucrose does not chemically react with cell organelles Due to the density and size of sucrose molecules‚ it is able to suspend pellets for configuration while providing a solution where the centrifugation can be better balanced Sucrose offers a liquid medium in which less dense fractions can be poured off as supernatant at the end of each centrifugation step. 0.25M sucrose solution
Free Buffer solution