Aseptic Technique and Culturing Microbes Lab 3 Brittney Case August 4th 2013 Section: Mic 101 Microbiology Session 1 Abstract: The Purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures‚ learning about cultured media and how to distinguish various types of microbial growths as well as what is required for them to grow properly. Hypothesis: This exercise will allow me to gain knowledge of how to properly use aseptic techniques
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Kaur Pure Cultures Lab 1/31/13 Introduction : Pure cultures are made of only one type of organisms and can be used to study their properties. A method used to isolate pure cultures is making a steak-plate‚ which is a dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod‚ culture was taken from the tube and dragged across area 1 several time‚of the agar. The agar was then turned 90º‚ and the loop was flamed and cooled. Taking some culture from
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ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and
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Aseptic Technique is based on surgical conscience; that is‚ the ethical and professional motivation that regulates a professional’s behaviors regarding disease transmission. (Fuller) All patients are bound to get an infection. Certain situations can increase vulnerability‚ like disturbance of the body’s defenses like contradictions to anesthesia‚ severe burns or an immune disorder. A key difference between the operating room and other clinical environments is that the operating area has high
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absence of bacteria‚ fungi‚ viruses or other microorganisms that could cause disease. Aseptic techniques refer to a set of skills that are used to ensure any environment being examined or dressed remains free from micro-organisms by not directly touching the wound or any other surface that might come into contact with the wound (McFerran and Martin 2008). Sterilization techniques and effective hand washing technique also play a major role in maintaining aseptic conditions. Aseptic techniques may be
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Abstract: Bacteria consists of a large domain of prokaryotic microorganism in which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly
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Aseptic Techniques Karla Brown HCP/210 October 11‚ 2013 Aseptic Techniques The supplies and equipment used to prepare sterile products are the following according to (The Pharmacy Technician: A Comprehensive Approach‚ Second Edition‚ ch.22‚ Pg. 611). • . NS—0.225% Sodium Chloride • D5.NS—5% Dextrose and 0.45% Sodium Chloride (for injection‚ USP) • 70% isopropyl alcohol—for cleaning surfaces • Alcohol pads—for cleaning ports‚ stoppers‚ skin surfaces‚ etc. • D10W—10% Dextrose in Water
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Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters
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ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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Aseptic technique Michele Pearson‚ Leah Christine Silver‚ and William Jarvis Purpose Aseptic technique is employed to maximize and maintain asepsis‚ the absence of pathogenic organisms‚ in the clinical setting. The goals of aseptic technique are to protect the patient from infection and to prevent the spread of pathogens. Often‚ practices that clean (remove dirt and other impurities)‚ sanitize (reduce the number of microorganisms to safe levels)‚ or disinfect (remove most microorganisms but not
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