investigated compounds were dissolved in DMSO at 10 and 20 mg ml-1‚separately [19‚21‚25‚27‚34]. Nutrient agar was prepared‚ then sterilized in an autoclave and poured in sterile Petri plates. After cooling of nutrient ager in Petri plates‚ the studied organisms were grown on agar. After that‚ the sterili Paper discs of Whatman saturated with solution of investigated compounds were placed in the agar by working holes using a sterile crook borer. These Petri dishes were incubated for 24 h at 37oC [25]
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Lab Practicum‚ Micro 200‚ study guide When you did your hand wash experiment‚ you decided a variable to test‚ say‚ hot vs cold water‚ you called this variable an “experimental variable” since it was the variable you were testing experimentally. In order to get meaningful data‚ you need to keep other things the same‚ for example the washing time had to be the same for both‚ you called the washing time a _Control_______ variable. Through the semester‚ you have used two bacteria that produce
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fermentation vs. respiration of microorganisms. One such medium‚ Thioglycollate Broth is useful for determining the oxygen relationship of a microorganism. The medium contains thioglycollic acid‚ cystine and agar‚ among other things. (Anaerobic Organisms Wikipedia).The thioglycollic acid and agar prevent oxygen from entering the entire medium. The medium used was a plate of TSA +glucose‚ one incubated anaerobically in a
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The first experiment involved agar cubes‚ each with a different surface area‚ to measure how surface area affects the rate of diffusion. In this experiment‚ the constants included 2% agar containing the pH indicator dye phenolphthalein‚ 1% phenolphthalein solution‚ 0.1M NaOH‚ 0.1M HCl‚ squares of hard‚ thin plastic (from disposable plates); unserrated knives; or scalpels from dissection kits‚ metric rulers‚ petri dishes or test tubes to hold the agar cubes. For the independent variable‚
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discovered by Lederberg and Tatum in 1946. In their experiment‚ they grew two strains of bacteria in separate vessels with rich medium and then together in one vessel containing the same medium. Then‚ they spread the three vessel contents onto medium agar plates and incubated them overnight at 37˚C. The only plate that showed cell growth was the plate containing the mixture of the two bacterial strains. The other two plates showed no growth. This experiment proved that in order for recombination to
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For experiments 1and 2‚ many different tests were conducted in order to investigate the different microbes found on the different types of turkey sampled. To begin‚ our group obtained 5 different forms of turkey that are encountered in everyday life: Inspirations deli turkey from Hannaford supermarkets‚ turkey bacon‚ turkey jerky from Whole Foods‚ Inspirations ground turkey from Hannaford‚ and a raw whole chunk slice of turkey breast form Whole Foods‚ these foods were considered experiment 1. Experiment
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O Revenue/Yield Management no transporte aéreo [pic] ISEC – Licenciatura em Gestão Aeronáutica ECONOMIA E GESTÃO DO TRANSPORTE AÉREO II 3º ano 2009/2010 Trabalho de Grupo realizado por: Aurora Carvalho Carlos Roquette Rui Gonçalves Índice Introdução 3 1. Conceitos essenciais do Revenue/Yield Management 3 2. O Revenue/Yield Management no transporte aéreo 7 3. Implementação de um programa de Revenue/Yield Management 11 4. Análise
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Materials • 30 petri dishes • Nutrient agar (a culture containing agar used to grow bacteria) • Sterile paper disks • Bacillus subtilis broth • Home-built incubator (a sealed box with a heat pad underneath) • Light box • Penicillin • Streptomycin • Tetracycline • Glutaraldehyde • 5 percent phenol alcohol • Fresh garlic extract Procedures 1. Divide petri dishes into six groups of five. 2. With adult supervision‚ melt the agar medium. 3. When liquid agar cools‚ pour an equal amount into each
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Work Plan for Isolation‚ Purification‚ Identification and Starter Culture Activity of Lactoccocus lactis Submitted by: M.Usman Akram B.S. (Hons.) Dairy Technology mh.usman@hotmail.com Mobile : +923217773736 University of Veterinary and Animal Sciences‚ Ravi Campus Pattoki Lactoccocus lactis Classification: Scientific classification | Kingdom: | Bacteria | Division: | Firmicutes | Class: | Bacilli | Order: | Lactobacillales | Family: | Streptococcaceae | Genus:
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Historical synonym: Bacillus difficile. Morphology Gram-positive‚ straight rods‚ motility is variable‚ have a flagella. Spores are oval‚ sub terminal swelling the cell. Cultural characteristics Colonies on blood agar plates are circular‚ flat or low convex‚ opaque‚
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