WUS101/2 TERAS KEUSAHAWANAN SEM II 2012/2013 LAPORAN PROFIL USAHAWAN Prepared by: NORHAMIZAH BINTI IDROS 108965 930623-10-7864 KEJURUTERAAN ELEKTRONIK (EE) Prepared for: MOHD KAMIL ARIFFIN LECTURER WUS101 USM 25 APRIL 2013 Date: 20 APRIL 2013 Norhamizah Idros Kampus Kejuruteraan Universiti Sains Malaysia 14300 Nibong Tebal‚ Pulau Pinang. Mohd Shafie Ariffin Lecturer of WUS101 Universiti Sains Malaysia. Submission of WUS101
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of bacteria in a given cultured sample is too great to be counted directly. The samples will be serially diluted by a factor of 10 in sterile saline‚ to be repeated 7 times. A 10 microliter aliquot from each dilution will be placed on a 5% blood agar plate or a MacConkey plate‚ depending on the type of organisms plated. These plates are selected because of the ease of differentiation between organisms. The original vial and all diluted aliquots will then be refrigerated to restrict growth until
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technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile filter paper discs (using a disc dispenser)‚ which contain a known concentration of antibiotics. As soon as the antibiotic discs touch the agar‚ the antibiotic will begin to diffuse into the surrounding agar. During incubation the bacteria you inoculated onto the agar
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procedure performed was an isolation of my unknown bacteria with the goal of obtaining a pure culture. This was done by streaking the unknown onto a nutrient agar plate using the streak method. The plates were incubated for two days and the bacterium was able to grow. I studied the bacteria based of its physical characteristics of how it grew on the agar. I began my quest by conducting a Gram stain on my bacteria. I prepared a smear by placing a drop of water onto the center of a slide and then removed
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small end bent to close it. * 1 Sharpie marking pen. * 1 glass test tube with a cap containing 2 ml of sterile nutrient broth and labeled "Broth". * 2 Petri dishes containing only nutrient agar and labeled "No Amp" on the bottom with date. * 2 Petri dishes containing nutrient agar and the antibiotic ampicillin. The dishes should be labeled "Amp" on the bottom with the date. * The laboratory instructions. Methods: Pre-Lab: * PREPARATION OF THE E. COLI STARTER
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respectiv a unui circuit de amplificare cu AO‚ este descrisă în setul de probleme alocat cursului 10 şi se bazează pe caracteristica de funcţionare a AOului‚ respectiv a circuitului. a. Caracteristica de funcţionare a AOului este indicată în figura de mai jos. Intervalul de valori al tensiunii vID care corespunde regiunii liniare este reprezentat de intervalul de valori [VLmin ÷ VLmax]. Caracteristica de funcţionare a AOului. În cazul în care valoarea în modul a celor 2 surse de alimentare este egală
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This is a quantitative test and is considered to be the gold-standard method for biofilm detection (13). Organisms isolated from fresh agar plates were inoculated in 10 mL of trypticase soy broth with 1% glucose. Broths were incubated at 370C for 24 hours. The cultures were then diluted 1:100 with fresh medium. Individual wells of sterile 96 well-flat bottom polystyrene tissue culture
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Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then the bacteria is non-hemolytic. For my bacteria no change is observed in the media therefore the bacteria is non-hemolytic and negative result. MacConkey Agar test
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Digital image. Flickriver.com. Flickriver Viewer Software‚ 2007. Web. 3 Nov. 2013. An endospore stain of Bacillus cereus using malachite green. Digital image. Academic.missouriwestern.edu. N.p.‚ n.d. Web. 3 Nov. 2013. Hahn‚ Brittany. Phenyl Ethyl Alcohol agar. Digital image. Studyblue.com. STUDYBLUE INC.‚ 22 Apr. 2013. Web. 3 Nov. 2013. Reynolds‚ Jackie. Selective and differential media. Digital image. Www.microbelibrary.org). Kern Community College District‚ 12 Sept. 2011. Web. 3 Nov. 2013. Rose‚ Scott
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was in order to test the many effects of diffusion and osmosis amongst four experiments. One such experiment was testing the effects of molecular weight on diffusion in relation to the use of Agar. The methods performed included the use of two acids‚ HCl and acetic acid. Both acids were placed into an Agar-filled dish and‚ over increments of 15 minutes‚ data collection was taken based off the diffusion rate and the diameter length of both the HCl and the Acetic Acid. The resulting factor was the
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