PEX 04 02

Name: Vargas,Aida 12
Exercise 4: Endocrine System Physiology: Activity 2: Plasma Glucose, Insulin, and Diabetes Mellitus Lab Report
Pre-lab Quiz Results
You have not completed the Pre-lab Quiz.

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page 1

Experiment Results
Predict Question:
Predict Question: To measure the amount of plasma glucose in a patient sample
Your answer : b. the optical density of the sample will be measured and the glucose concentration will be extrapolated from the glucose standard curve.
Stop & Think Questions:
Why does the color in the tubes vary?
You correctly answered: c. The tubes contain different amounts of glucose.
Experiment Data:

Part 1

Part 2

Tube
1
2
3
4
5
1
2
3
4
5

Optical Density
0.30
0.50
0.60
0.80
1.00
0.73
0.79
0.89
0.83
0.96

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page 2

Glucose (mg/deciliter)
30
60
90
120
150
150
120
120
90
150

Post-lab Quiz Results
You scored 100% by answering 6 out of 6 questions correctly.
1. A male patient has had successive fasting plasma glucose readings of 115, 110, and 122 mg/dl. The healthcare provider will inform him that
You correctly answered: c. he appears to have impairment or borderline impairment of insulin-mediated glucose uptake by his cells.
2. To obtain an accurate spectrophotometric measurement of the glucose concentration in the sample
You correctly answered: d. heparin is added to prevent blood clots.
3. In the spectrophotometric assay used in this experiment, the __________ as the glucose concentration in the sample increases. You correctly answered: b. optical density increases
4. A female patient has had successive fasting plasma glucose readings of 130, 140, and 128 mg/dl. The healthcare provider will inform her that
You correctly answered: a. she has developed diabetes.
5. To maintain plasma glucose homeostasis
You correctly answered: d. insulin-mediated transport of glucose into cells acts as negative feedback when plasma glucose levels rise.
6. A laboratory technician withdraws a blood sample from a vein in your upper arm knowing that