Investigating the oral glucose tolerance test
Aim: To carry out glucose tests on stimulated blood plasma samples if glucose is present in blood plasma. Risk Assessment:
Hazardous chemical or microorganism being used or made, or hazardous procedure or equipment.
Nature of the hazard(s)
Source(s) of information
Control measures to reduce the risks Sulphuric acid
- Very corrosive
Wear eye protection & wear lab coat. Try not to spill chemical. Potassium manganate
- Harmful over 1 ml
- Danger to environment
- Oxidising agent
Wear lab coat & wear eye protection. Wash hands thoroughly at the end of the experiment. Glassware
(glass stirring rod, boiling tubes)
- Can break if dropped and could cause bleeding or other damage to skin.
Experience in situation
Try not to drop glassware and wear eye protection. Keep working area clear at all times.
Measure 10cm3 of 4 mol dm-3 glucose solution into the first boiling tube 2)
Measure 5cm3 of 1 mol dm-3 sulphuric acid and add to the boiling tube from 1 3)
Reset the stop clock
Use a dropping pipette to take 2cm3 potassium manganate VII solution from a measuring cylinder 5)
Add the potassium manganate to the boiling tube and starting the stop clock at the same time 6)
Stir the contents of the boiling tube with a stirring rod and stop the clock as the solution becomes colourless 7)
Record the time taken to go colourless in a results table 8)
Wash boiling tube and dropping pipettes thoroughly
Repeat steps 2-8 for the remaining glucose solutions.
Time to decolourise
(Graph of results on next page)
Time for glucose
Time for glucose
Interpretation of Results:
From the graph of my results I can see that...
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