Brent Good Unit 3 Lab
LABORATORY REPORT
Activity:
Blood Glucose Regulation
Name:
Brent Goo
Instructor:
Thomas Redenbaugh
Date:
12.23.2014
Predictions
Plasma glucose levels will be highest
1-3 hours after the meal
Plasma ketone levels will be highest before the meal (fasting)
Plasma insulin levels will be highest immediately after the meal (0 hr)
Plasma glucagon levels will be highest
1-3 hours after the meal
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
Patients were advised not to consume caffeine and alcohol or participate in any physical activities before the test.
5. What type of assay was used to measure plasma glucose and ketone levels?
Enzymatic analysis
6. What type of assay was used to measure plasma insulin and glucagon levels?
Radioimmunossary
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.3
6.4
6.4
5.5
4.8
Glucose (mmol/l)
Sub. 2
Sub. 3
5.3
5.2
6.4
6.4
6.3
5.6
4.7
6.4
5.6
4.7
average
5.3
6.4
Sub. 1
105
114
6.4
5.6
4.7
50
66
74
Laboratory Report/ Brent Goo/ Blood Glucose Regulation/ Thomas Redenbaugh/ 12.23.2014/ Page [1] of [4]
Ketones (µmol/l)
Sub. 2
Sub. 3
111
113
88
94
54
62
74
76
75
46
average
110
99
60
68
65
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
0 hours post meal - 1 hour post meal
2. When was plasma glucose concentration lowest? If values are similar for several time points, then give range of times when plasma glucose concentration was
Activity:
Blood Glucose Regulation
Name:
Brent Goo
Instructor:
Thomas Redenbaugh
Date:
12.23.2014
Predictions
Plasma glucose levels will be highest
1-3 hours after the meal
Plasma ketone levels will be highest before the meal (fasting)
Plasma insulin levels will be highest immediately after the meal (0 hr)
Plasma glucagon levels will be highest
1-3 hours after the meal
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
Patients were advised not to consume caffeine and alcohol or participate in any physical activities before the test.
5. What type of assay was used to measure plasma glucose and ketone levels?
Enzymatic analysis
6. What type of assay was used to measure plasma insulin and glucagon levels?
Radioimmunossary
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.3
6.4
6.4
5.5
4.8
Glucose (mmol/l)
Sub. 2
Sub. 3
5.3
5.2
6.4
6.4
6.3
5.6
4.7
6.4
5.6
4.7
average
5.3
6.4
Sub. 1
105
114
6.4
5.6
4.7
50
66
74
Laboratory Report/ Brent Goo/ Blood Glucose Regulation/ Thomas Redenbaugh/ 12.23.2014/ Page [1] of [4]
Ketones (µmol/l)
Sub. 2
Sub. 3
111
113
88
94
54
62
74
76
75
46
average
110
99
60
68
65
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
0 hours post meal - 1 hour post meal
2. When was plasma glucose concentration lowest? If values are similar for several time points, then give range of times when plasma glucose concentration was