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Micropropagation of Labisia Pumila using apical meristem
INTRODUCTION
Labisia pumila belongs to the family Myrsinaceae and the common name (in Malaysia) is Kacip Fatimah. There are three main varieties of Labisia pumila found in Malaysia var. pumila, var. alata and var. lanceolata. Labisia pumila var alata has long being recognized by Malay women for its medicinal value. It is used to induce and facilitate childbirth as well as a post partum medication to help contract the birth channel, to tone the abdominal muscles and to regain body strength (Wan Ezumi et al., 2007). Other traditional uses include treatment for dysentery, rheumatism and gonorrhea. L. pumila products are widely available commercially as health supplements. In spite of the wide usage of this plant as a traditional herb no information on its chemical constituents has been documented. However, it was reported that plants from the same genus (Myrsinaceae) are being used to treat respiratory tract infections and menstrual disorders (Huang et al., 2000). This plant is mostly obtained from the natural tropical forest. Its availability in the forest is becoming scarce due to the increase in demand by the users (Satefarzi 2000). In addition, its growth rate in the natural habitat is very slow (Mohd Noh et al. 2002). Logging activities and encroachment of the forests have also contributed to the problem. It is anticipated that this plant species will face extinction and severe genetic loss if necessary steps are not taken to replant it. To avoid further depletion of this species, there is a need to domesticate and cultivate it Therefore, suitable technique for mass propagation has to be developed. Plant tissue culture technology may help to conserve rare and endangered medicinal plants. Many important Chinese medicinal herbs have been successfully propagated in vitro, either by organogenesis (Erdei et al. 1981, Shoyama et al. 1983, Hatano et al. 1986, Matsumoto et al. 1986, Hiraoka and Oyanagi 1988, Nishioka 1988, Tsay et al. 1989, Huang et al. 2000, Chen et al. 2001, Chueh et al. 2001) or by somatic embryogenesis (Hiraoka et al. 1986, Kitamura et al. 1989, Tsay and Huang 1998, Sagare et al. 2000). In vitro propagated plants of many important medicinal specieswere found to be uniform, showing less variation in their content of secondary metabolites than their wild/cultivated counterparts (Yamada et al. 1991).

RESEARCH PROBLEM
Low fruit set ratio of Labisia Pumila in wilderness cause a breeding program need to be developed to handle this program. RESEARCH HYPHOTHESIS
Regeneration of Labisia pumila through shoot tip culture can be achieved.

OBJECTIVE
1. To initiate shoots from apical meristem.
2. To establish rooted plantlet of labisia pumila.

LITERATURE REVIEW
1. Labisia pumila var alata (Kacip Fatimah)
Labisia pumila belongs to the family Myrsinaceae and the common name (in Malaysia) is Kacip Fatimah. There are three main varieties of Labisia pumila found in Malaysia var. pumila, var. alata and var. lanceolata. Labisia pumila var alata has long being recognized by Malay women for its medicinal value. Traditionally are used mostly before childbirth in inducing and ease delivery and as postpartum medication to help contract the birth channel, to delay re-conception and to regain body strength. Kacip Fatimah benefits women in several ways. These benefits help care for a woman's healthy sex life. Female libido enhancement is the end result of optimum sexual health. It is also believed to be beneficial in firming and toning of abdominal muscles, breasts and tighten vaginal muscles, anti-dysmenorrhoea, cleansing and avoiding painful or difficult menstruation, to treat dysentery, rheumatism and women's ailments associated with childbirth (Burkill, 1966). Usually the plant is boiled and the water extract is than taken.

2. Kacip Fatimah propagation

Traditionally, the methods that are employed to propagate Labisia Pumila are...
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