Biofilm-Mediated Enhanced Crude Oil Degradation by

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International Research Journal of Environment Sciences________________________________ ISSN 2319–1414 Vol. 2(2), 48-52, February (2013) Int. Res. J. Environment Sci.

Isolation, Characterization and Identification of Diesel Engine Oil Degrading Bacteria from Garage Soil and Comparison of their Bioremediation Potential Teli Nikhil1, Verma Deepa2, Gavankar Rohan1 and Bhalerao Satish3 1

Department of Biotechnology, Viva College, Virar (W), Maharashtra, INDIA 2 Department of Botany, Viva College, Virar (w), Maharashtra, INDIA 3 Department of Botany, Wilson College, Mumbai, Maharashtra, INDIA

Available online at: www.isca.in
Received 30th November 2012, revised 12th January 2013, accepted 25th January 2013

Abstract
The rate of biodegradation of diesel engine oil by microorganisms isolated from garage soil (petroleum contaminated soil) was studied. Modified diesel engine oil medium was used and two most abundant microorganisms were isolated from garage soil – Micrococcus sp. and Pseudomonas sp. were found to be hydrocarbon degraders and these two bacteria’s were selected for the degradation test. The degradation of diesel engine oil was monitored at a five day interval up to twenty five day period, using gravimetric method. After 25 days of incubation period, Pseudomonas sp. degraded 67.57 % of the oil and Micrococcus sp. with 52.95 %. But the mixture of Micrococcus sp. and Pseudomonas sp. were found to have great potential to degrade diesel engine oil i.e. 89.98 % after 25 days. The rate of degradation of diesel engine oil by Micrococcus sp. was found to be 7.48 x 10-4gm/hr and that of Pseudomonas sp. was 9.55 x 10-4gm/hr while the mixture of both bacterial isolates showed highest rate of degradation of diesel engine oil i.e. 1.27 x 10-3gm/hr. Keywords: Bioremediation, Diesel engine oil, oil spills, hydrocarbon degraders, Micrococcus sp., Pseudomonas sp.

Introduction
As we dig deeper into the modern industrial age of technologies, several aspects of human life change. People benefit largely from life development and many live in prosperity, but prosperity has a price. This price is paid by our environment that suffers daily from all kinds of pollutants and destruction. People now have to find ways to cure this destruction. Oil contamination is one of the most dangerous pollution factors known today. It can cause a threat to the environment. It is very feared by environmentalists and it's very hard to control if it gets out of hand. Oil spills have been a major issue across decades. One of the famous oil spills which are also ongoing is in Taylor Energy Well in Gulf of Mexico, U.S.A caused due to Hurricane; Sept 16, 2004 till present date and almost 0.03- 0.05 tones oil/per day is estimated to leak. Another recent oil spill was in Mumbai (India) and caused due to the leakage in Mumbai-Uran pipeline dated January 21, 2011 and about 55 tons of oil was leaked in Arabian Sea. Various such accidents occur throughout the years and it causes damage to our surrounding. Diesel engine oil, which is one of the major products of crude oil, constitutes a major source of pollution in our environment. With the combined dependence on diesel engine oil by some vehicles and generators, greater quantities are being transported

over long distances. Therefore diesel engine oil can enter into the environment through wrecks of oil tankers carrying diesel oil, cleaning of diesel tanks by merchants, war ships carrying diesel oil and motor mechanics1. Diesel oil spills on agricultural land generally reduce plant growth. Suggested reasons for the reduced plant growth in diesel oil contaminated soils range from direct toxic effect on plants2 and reduced germination to unsatisfactory soil condition due to insufficient aeration of the soil because of the displacement of air from the space between the soil particles by diesel engine oil3. Among several clean–up techniques available to remove petroleum hydrocarbons from the soil and...
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