enzymes
LABORATORY REPORT
Activity: Enzyme Activity
Name: Angela Collins
Instructor: Catherine Rice
Date: 07.09.2014
Predictions
Sucrase will have the greatest activity at pH 5
Sucrase will have the greatest activity at
70 °C (158 °F)
Sucrase activity increases with increasing sucrose concentration
Materials and Methods
Effect of pH on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable pH Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable temperature Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable amount of substrate (sucrose) present
Controlled Variables temperature, pH, sucrase + sucrose incubation time
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. the optimal density is measured because it measures the amount of glucose and fructose produced.
5. Explain why denatured sucrase was used as a control. because denature sucrose will not have as much activity as the other sources.
Laboratory Report/ Angela Collins/ Enzyme Activity/ Catherine Rice/ 07.09.2014/ Page [1] of [4]
6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration.
Results
Table 1: Effect of pH on Sucrase Activity
Optical Density ph 3 ph 4 ph 5 ph 6 ph 7 ph 8 ph 9
1 0.189 0.308 0.674 0.943 0.772 0.417 0.164
2 0.187 0.294 0.654 0.961 0.817 0.409 0.184
3 0.173 0.318 0.642 0.928 0.825 0.398 0.161 average 0.183 0.307 0.657 0.944 0.805 0.408
Activity: Enzyme Activity
Name: Angela Collins
Instructor: Catherine Rice
Date: 07.09.2014
Predictions
Sucrase will have the greatest activity at pH 5
Sucrase will have the greatest activity at
70 °C (158 °F)
Sucrase activity increases with increasing sucrose concentration
Materials and Methods
Effect of pH on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable pH Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable temperature Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity
Dependent Variable amount of product (glucose and fructose) produced
Independent Variable amount of substrate (sucrose) present
Controlled Variables temperature, pH, sucrase + sucrose incubation time
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. the optimal density is measured because it measures the amount of glucose and fructose produced.
5. Explain why denatured sucrase was used as a control. because denature sucrose will not have as much activity as the other sources.
Laboratory Report/ Angela Collins/ Enzyme Activity/ Catherine Rice/ 07.09.2014/ Page [1] of [4]
6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration.
Results
Table 1: Effect of pH on Sucrase Activity
Optical Density ph 3 ph 4 ph 5 ph 6 ph 7 ph 8 ph 9
1 0.189 0.308 0.674 0.943 0.772 0.417 0.164
2 0.187 0.294 0.654 0.961 0.817 0.409 0.184
3 0.173 0.318 0.642 0.928 0.825 0.398 0.161 average 0.183 0.307 0.657 0.944 0.805 0.408