Factors That Affect the Rate of Reaction of Peroxidase

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Purpose:
To determine the effect of various factors on the rate of reaction between an enzyme andits substrate, and also to determine the optimal ranges under which the enzyme activity ismaximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis:

PH factor prediction: I predict that as the pH increases so the activity of the enzyme willincrease until it reaches optimum pH range (pH 7) because the enzyme is less denaturedwhen it reaches the preferred pH level, and after this it will decrease because the activesite will change in shape and it will no longer accept substrates.Temperature factor prediction: I predict as the temperature increases, the enzymeactivities will increase because there is more energy to speed up the reaction until itreaches the optimum temperature range (room temperature which is about 20 °C), andafter that the enzyme activities will decrease because of denature of the enzymes (causechanges to active site that will no longer fit substrate)Concentration of enzymes prediction: I predict that as the concentration of enzymeincreases, so the enzyme activities will increase because there is more enzyme to reactwith the substrates however when enzymes get saturated, the reaction will come to a plateau because eventually all the substrates will have enzymes to react with, and anyextra will have no effect on the reaction whatsoever.I predict alcohol is an inhibitor of Peroxidase because alcohol when alcohol bind to theallosteric site it changes the active site shape of the enzymes thus deactivating enzymaticactivitiesI predict salt is an activator of Peroxidase because salt contains Na ions which attaches tothe allosteric site changing the shape of the enzyme to fit a substrate. Materials:


Peroxidase (enzyme in potato)

Hydrogen peroxide, 3%

A strong acid, pH3 (lemon juice, or HCL) 0.5

A strong base, pH 10 (drain cleaner, NaOH) 0.5 mol/L

A weak acid, pH 6 (vinegar, acetic acid( CH3COOH)) 0.5...
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